Confocal Raman Spectroscopy: in Vivo Measurement of Physiological Skin Parameters

Confocal Raman Spectroscopy: in Vivo Measurement of Physiological Skin Parameters - A Pilot Study

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02854475

Enrollment

Registered

2016-08-03

Start date

2016-07-31

Completion date

2016-07-31

Last updated

2016-08-03

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Dermatologic Disorders, Disorder of Cholesterol Metabolism

Keywords

skin parameters, cholesterol, Raman spectroscopy

Brief summary

The purpose of this study is to I. determine several skin parameters, for example natural moisturizing factor (NMF) and cholesterol, with Raman spectroscopy in vivo and II. compare the results with the corresponding cholesterol levels measured in venous blood.

Detailed description

In vivo Raman experiments will be carried out weekly on voluntary participants using a RiverDiagnostics gen2-SCA Skin Composition Analyzer (RiverDiagnostics, Rotterdam, Netherlands). All experiments will be carried out on the volar forearm. Semi-quantitative concentration profiles for several skin parameters will be determined, for example natural moisturizing factor (NMF) and cholesterol. Additionally the total cholesterol level will be detected by venous blood sample analysis. The blood samples will be taken weekly within two weeks, similar to the Raman measurements.

Interventions

PROCEDUREVenous blood collection

weekly within two weeks

PROCEDURERaman spectroscopy

weekly within two weeks, on the volar forearm, using a RiverDiagnostics gen2-SCA Skin Composition Analyzer

Study design

Allocation

Intervention model

SINGLE_GROUP

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

18 Years to 65 Years

Healthy volunteers

Yes

Inclusion criteria

* Provision of subject informed consent * Female or male aged between 18 and 65 years

Exclusion criteria

* Pregnancy * Chronic skin conditions

Design outcomes

Primary

Measure	Time frame
Correlation of the fasting total cholesterol levels measured in skin with confocal Raman spectroscopy and in venous blood collected by venipuncture	2 weeks

Secondary

Measure	Time frame
Concentration curves of various skin substances, such as NMF, urea and water as evidence for the eligibility of confocal Raman spectroscopy for the in vivo measurement of skin	2 weeks

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026