Normal Subjects
Conditions
Keywords
sweeteners, sugar, EEG, food viewing, food intake
Brief summary
This study aims at investigating the acute influence of artificially sweetened beverages consumption (as compared with sugar-sweetened beverages and water consumption) on brain responses to the viewing of food images, on physiological responses (gut-derived hormones and lipid metabolites) and on the food intake behavior.
Detailed description
This study is made on a randomized controlled crossover design including three conditions (water, sugar, artificial sweeteners). Measurements will be performed in fasting and fed conditions. The test-meal will comprise either an artificially or sugar-sweetened beverage or water (control). The spatio-temporal brain dynamics to the viewing of food pictures will be assessed by means of electroencephalography (EEG) recordings and electrical neuroimaging analyses. Venous blood and urine will be collected. Blood pressure, cardiac frequency and bio-impedance measurements will be performed. Food intake behavior will be assessed by means of questionnaires and a free-choice buffet. Each tested condition (water, sugar, artificial sweeteners) will be preceded by a run-in period during which the participants' diet will be controlled (2-day isoenergetic diet).
Interventions
OTHERingestion of a test meal with sweetener
participants will ingest a test meal containing 55% carbohydrate, 30% fat and 15% protein with artificially sweetened drinks
OTHERingestion of a test meal with water
participants will ingest a test meal containing 55% carbohydrate, 30% fat and 15% protein with waters
Sponsors
University of Lausanne Hospitals
University of Lausanne
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Caregiver, Investigator)
Eligibility
Sex/Gender
MALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy * Caucasian * Right-handed * Weak to normal physical activity (\< 3hours per week) * Normal-weight (19\<BMI\<25 kg/m2) * Regular consumption of sugar-sweetened beverages (\>33cl per week)
Exclusion criteria
* Consumption of artificially sweetened beverages exceeding 33cl per week * Diabetes * Cardiovascular, kidney, hepatic and/or psychological disorders * Blood pressure at rest \> 140/90mmHg * Body weight \< 50kg * Hemoglobin \< 13.5g/dl * Ferritin \< 50microg/l * Drug consumption * Alcohol consumption (\>10g/day) * Smoking * Any particular diet (e.g. vegetarianism), food allergy and/or intolerance * Body weight gain or loss of \>3kg during the last 3 months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Spatio-temporal brain dynamics | differences observed between 60 min before and 60 min after ingestion of a test meal | Spatio-temporal brain dynamics to the viewing of food will be assessed by means of electroencephalography recordings. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Changes in concentration of blood insulin | -120 min before ingestion of a test meal, and 30, 60, 90, 150, and 210 min after ingestion of a test meal | Blood substrate concentration will be measured in fasting conditions and after ingestion of a test meal |
| Changes in concentration of blood triglyceride | -120 min before ingestion of a test meal, and 30, 60, 90, 150, and 210 min after ingestion of a test meal | Blood substrate concentration will be measured in fasting conditions and after ingestion of a test meal |
| Changes in concentration of blood Fibroblast Growth Factor (FGF)-21 | -120 min before ingestion of a test meal, and 30, 60, 90, 150, and 210 min after ingestion of a test meal | Blood substrate concentration will be measured in fasting conditions and after ingestion of a test meal |
| spontaneous food intake | 240 min after ingestion of a test meal | Food intake behavior will be assessed by monitoring spontaneous food intake when offered a free-choice buffet. |
Countries
Switzerland
Outcome results
None listed