Physiology
Conditions
Brief summary
NO is an ubiquitous molecule contributing to many physiological functions such as vasodilation, immune and oxidative stress responses. NO production can be supplemented either by providing substrate to the NO-synthase (L-Arginine and L-Citruline) or by providing nitrate which can lead to NO. In the present project the investigators evaluate the effect of various acute and chronic oral NO precursors supplementation on exercise performance and responses in healthy young and older subjects.
Interventions
DIETARY_SUPPLEMENTNitrate
Subjects will be supplemented orally either acutely or chronically (4-8 weeks) with NO precursors.
DIETARY_SUPPLEMENTNitrate and L-Arginine
Subjects will be supplemented orally either acutely or chronically (4-8 weeks) with NO precursors.
DIETARY_SUPPLEMENTNitrate and L-Citrulline
Subjects will be supplemented orally either acutely or chronically (4-8 weeks) with NO precursors.
DIETARY_SUPPLEMENTPlacebo
Subjects will be supplemented orally either acutely or chronically (4-8 weeks) with a placebo.
BIOLOGICALEvaluation of post-ischemic vascular reactivity
After the intake of the supplement. Concentration of hemoglobin (g/mL) in the quadriceps with the device : NIRS system Oxymon, ARTINIS.
BIOLOGICALNeuromuscular evaluations
After the intake of the supplement. Measure the stretching force in the ankle (N)
Sponsors
University Hospital, Grenoble
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* Sedentary subjects * No cardiovascular, respiratory, metabolic and neurological disorders
Exclusion criteria
* Body mass index \> 28 kg.m-2 * Smoker * Alcool intake \> 10g/day * Psychiatric diseases * Subjects under treatment able to interfere with their cardiovascular, metabolic and neurological status
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Neuromuscular evaluations | 3 half days | After the intake of the supplement. Measure the stretching force in the ankle (N) |
| Evaluation of post-ischemic vascular reactivity | 3 half days | After the intake of the supplement. Concentration of hemoglobin (g/mL) in the quadriceps with the device : NIRS system Oxymon, ARTINIS. |
Countries
France
Outcome results
None listed