Expression of miRNa-138 and Cyclin D1 in Oral Mucosa of Patients With Oral Lichen Planus

Expression of miRNA-138 and Cyclin D1 in Oral Lichen Planus (A Study Utilizing Immunohistochemistry and Real-time Polymerase Chain Reaction)

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02834520

Enrollment

Registered

2016-07-15

Start date

2015-05-31

Completion date

2016-03-31

Last updated

2016-07-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Oral Lichen Planus

Brief summary

60 individuals were subdivided into 2 groups, 30 patients with oral lichen planus, 30 control individuals. Expression of miRNA-138 and cyclin D1 were evaluated in oral mucosa utilizing Quantitative real-time polymerase chain reaction and immunohistochemistry.

Detailed description

60 individuals were subdivided into 3 groups, into 2 groups, 30 patients with oral lichen planus, 30 control individuals having age, gender and periodontal status matched with both patients groups acting as a control group. Relative gene expression of miRNA-138 was estimated using Quantitative real-time polymerase chain reaction technique in oral mucosa of both oral lichen planus patients and control subjects. Sub grouping of oral lichen planus patients were performed into reticular, atrophic and erosive groups (n=10 each). Cyclin D1 gene expression was estimated using Quantitative real-time polymerase chain reaction technique and Cyclin D1 protein level was estimated using immunohistochemistry.

Interventions

None listed

Study design

Observational model

CASE_CONTROL

Time perspective

CROSS_SECTIONAL

Eligibility

Sex/Gender

ALL

Age

45 Years to 65 Years

Healthy volunteers

Yes

Inclusion criteria

* patients with oral lichen planus.

Exclusion criteria

* any systemic disease other than oral lichen planus. * smokers. * pregnant females. * periodontal diseases.

Design outcomes

Primary

Measure	Time frame	Description
expression of miRNA-138	1 day	expression of miRNA-138 in oral mucosa of patients with oral lichen planus

Secondary

Measure	Time frame	Description
gene expression of cyclin D1	1 day	gene expression of cyclin D1 in oral mucosa of patients with oral lichen planus
protein expression of cyclin D1	1 day	protein expression of cyclin D1 in oral mucosa of patients with oral lichen planus

Outcome results