Ischemia, Peripheral Artery Disease
Conditions
Brief summary
The purpose of this study is to evaluate the effect of muscle ischemia during claudication on mitochondrial function. Comparison to patients with arterial disease but absence of lower limb claudication (Cardiac group) feasibility study.
Detailed description
Assess the feasibility of the study of micro-vascular function and mitochondrial on muscle sample (sartorius) in a group of arterial disease and non arteriopathy group. Analysis of the activity of mitochondrial chain Immunochemistry of tissue markers
Interventions
OTHERMuscle Biopsy
A distal and proximal biopsy of the sartorius muscles ( 0.5 cm3 each) will be performed during surgery for saphenous vein withdrawal (cardiac group) or arterial bypass (PAD group) The samples are then treated with neurovascular and mitochondrial biology laboratory. the activity of the complex 1 is analyzed and compared between the 2 groups.
Sponsors
University Hospital, Angers
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Intervention for femoropopliteal bypass (PAD group) * Intervention for saphenous withdrawal for coronary bypass surgery (cardiac group)
Exclusion criteria
* Participate in another biomedical research * Pregnant woman
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Activity differences in mitochondrial complex I between the distal and proximal levy in group 1 and group 2. | 1 week | Measurement of mitochondrial complex 1 activity (arbitrary units) on muscle biopsies by the laboratory of biochemistry. Comparison of the results observed on the same miuscle at the proximal level (assumed normal in both groups) and distal level (assumed ischemic in claudicants but not in cardiac patients without limb symptoms. |
Countries
France
Outcome results
None listed