Scoliosis
Conditions
Keywords
Adolescent idiopathic Scoliosis, Ghrelin
Brief summary
Adolescent idiopathic Scoliosis (AIS) is the most common spine pathology. It is opposed to secondary scoliosis due to chronic diseases. Many hypotheses have been made to elucidate the origin of this illness. Recently, the melatonin pathway has been investigated as pinealectomy of the chicken creates a scoliosis that resembles AIS and melatonin supplementation reverses the process. In addition administration of melatonin to AIS patients improved the pathology. However this hypothesis has shown controversial results. Recent studies have demonstrated melatonin cellular resistance in osteoblastic cells from AIS patients. Melatonin acts through G protein coupled receptor (GPCR), mainly using the Gi pathway. In AIS osteoblasts, this pathway is blocked leading to a decrease in the inactivation of the adenylyl cyclase and therefore maintenance of high level of cyclic adenosine monophosphate (cAMP) concentrations in the cells. As modulation of cAMP is important for osteogenesis such resistance may be critical for the initiation or the development of AIS. Gi signalization is used by several other GPCR, thus, this hormonal resistance could logically be found in other hormonal or mediator pathways. A precedent study previously focused on ghrelin in AIS, and demonstrated that AIS patients possess elevated plasmatic values of ghrelin. This study also observed decreased response to ghrelin in AIS cultures osteoblasts.
Interventions
BIOLOGICALOsteoblast sample
culture osteoblasts obtained from vertebrae fragments from 30 patients with AIS and from 10 control patients with secondary scoliosis
Sponsors
University Hospital, Toulouse
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
12 Years to 15 Years
Healthy volunteers
No
Inclusion criteria
Group 1 : * AIS : spine deformity with angle above 10°,without detected cause * Informed consent obtained Group 2 : * Secondary scoliosis due to chronic illness, neurologic or syndromic, eligible for spine surgery * Spine surgery for reason other than scoliosis * Informed consent obtained
Exclusion criteria
* Not primary scoliosis * No consent * Legal obstacle
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Testing ghrelin cellular resistance by intracellular cAMP dosage | baseline | Testing ghrelin cellular resistance in AIS by measuring the variation of the intracellular cAMP level in response to ghrelin in osteoblast cells in vitro of AIS patients versus controls |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| ghrelin level in serum | baseline | Measure the ghrelin level in the serum of AIS patients versus controls |
| ghrelin levels in cellular supernatants | baseline | Measure the ghrelin levels in cellular supernatants of osteoblasts from AIS and controls patients in order to evaluate the ghrelin production of the cells. |
Countries
France
Outcome results
None listed