Colonic Neoplasms, Colonic Polyps
Conditions
Brief summary
The purpose of this study is to determine the effects of the plant hormones strigolactones, on cell cultures of colonic polyps and colorectal cancer.
Detailed description
Strigolactones have been shown to anti-proliferative and pro-apoptotic effects in cell cultures of breast and prostate origin, partially by down-regulation of cyclin B1 and induction of cell cycle arrest. In this study the investigators intend to take biopsies from benign colonic polyps and cancer tissue, culture intestinal explants and generate immortalized primary cells as a platform to assess the effect of this plant hormone on cell viability, cell mobility, apoptosis and cytoskeleton modifications.
Interventions
PROCEDUREbiopsy during colonoscopy
sampling the lesion with forcept biopter
Sponsors
Meir Medical Center
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Patients with positive findings during colonoscopy
Exclusion criteria
* Patients below 18 years old * Patients who cannot sign a consent form
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| percentage of cells with reduced viability after dose-dependent exposure | 24 hours of exposure to strigolactone | — |
| percentage of cells in G2/M arrest after dose-dependent exposure | 24 hours of exposure to strigolactone | analysis of cell cycle |
Contacts
Primary ContactIdo Laish, MD
Outcome results
None listed