Sleep Deprivation
Conditions
Keywords
Exercise training, Endothelial function
Brief summary
Rationale. Sleep loss is a risk factor for cardiovascular events mediated through endothelial dysfunction. Objective. To determine if 7 weeks of exercise training can limit cardiovascular dysfunction induced by total sleep deprivation (TSD) in healthy young men. Methods: 16 subjects will be examined during 40-h TSD, both before and after 7 weeks of interval exercise training. Vasodilatation induced by ACh, insulin and heat (42°C) as well as pulse wave velocity (PWV), blood pressure and heart rate (HR) will be assessed at baseline, during TSD, and after one night of sleep recovery. Biomarkers of endothelial activation, inflammation, and hormones will bemeasured from morning blood samples at 07:00
Interventions
OTHERSleep deprivation
40 hours of continuous wakefullness
Sponsors
Cetre du sommeil et de la vigilance, APHP
Institut de Recherche Biomedicale des Armees
Study design
Allocation
NON_RANDOMIZED
Intervention model
SINGLE_GROUP
Primary purpose
PREVENTION
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy * Men * 18 - 35 yrs
Exclusion criteria
* Consumming 400 mg of caffeine per day, * Body mass index (BMI) greater than 30 kg/m², * Taking medication. * Excessive daytime somnolence (Epworth Sleepiness Scale \>9) * Sleep complaints (Pittsburgh Sleep Quality Index \>5) * Inability to be considered as an intermediate chronotype on the Horne and Ostberg questionnaire (score \<31 or \>69) * Traines subject (Ricci Gagnon questionnaire \>35)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| ACh induced Vasodilation during TSD | Up to 09:00 during sleep deprivation (i.e. after 26 hours of wakefulness) | Cutaneous vasodilation (in % Baseline) induced by local application of Acethylcholine |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Insuline Vasodilation | Up to 09:00 during the sleep deprivation day and the recovery day | Cutaneous vasodilation (in % Baseline) induced by local application of insulin |
| Heat vasodilation | Up to 9:00 during the sleep deprivation day and during the recovery day | Cutaneous vasodilation (in % Baseline) induced by local application of heat (42°C) |
| Inflammation | Up to 7:00 during the sleep deprivation day and during the recovery day. | Plasma levels of inflammatory marquers (TNF-alpha, IL1beta...) |
| blood pressure | Every 3 hours during the 40 hours of wakefullness (i.e. total sleep deprivation) | Mean, systolic and diastolic blood pressure |
| PWV | Up to 09:00 during the sleep deprivation day | Pulse wave velovity evaluated by echography |
Outcome results
None listed