Infertility, Male
Conditions
Keywords
Male infertility, oxidative stress, antioxidants, probiotics, Vitamin E, Baker's Yeast
Brief summary
The purpose of this study is to evaluate the efficacy of Baker's yeast extractions , probiotic antioxidant, in scavenging the oxidative stress status mediated damage on sperm motility,progressive motility and vitality as well as comparing this potency to that of Vitamin E in infertile men.
Detailed description
The practical part of the study took place from August 2020 to November 2021. The Study population was made up of infertile males attending Hawaa fertility center, banha city, Qaliubiya, Egypt. 40 study samples were collected from patients who indicated willingness to participate in the study and have had 3 to 7 days of sexual abstinence, using the masturbation method and ejaculated in wide mouthed plastic container as described by World Health Organization (WHO). Samples were allowed to liquefy for 20 minutes and were examined for volume, viscosity, spermatozoa count by Neubauer haemocytometer , oxidative stress level measurement by Oxisperm, percentage of progressive motility and non-progressive motility and sperm vitality. The Semen specimens were divided into ten equal fractions. First fraction was control, 0.1ml of liquefied semen mixed with 0.1ml Ham's F10 medium and incubated at 37o C for 30 minutes., 2nd: Vit. E, 3rd (Yeast extraction ( YE).Vitamin C was obtained from (Sigma, Germany), 3rd, 4th, 5th fractions (Vit. C. 1,2,3), 0.1ml liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (0.02, 0.04, 0.06 mg/ml) Vitamin C respectively and incubated at 37o C for 30 minutes. After semen specimen treated in all fractions, they were examined and assessed for microscopical changes, total motility, progressive motility, vitality, level of oxidative stress (OS) were detected in all fractions 30 minutes later after addition of the antioxidants.
Interventions
DIETARY_SUPPLEMENTVit E
0.1ml liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (2 mg/ml) Vitamin E and incubated at 37o C for 30 minutes.
DIETARY_SUPPLEMENTYeast
0.1ml liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (20 mg/ml) yeast extraction and incubated at 37o C for 30 minutes.
DIETARY_SUPPLEMENTVit C 1
0.1ml liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (0.02 mg/ml) Vitamin C and incubated at 37o C for 30 minutes.
DIETARY_SUPPLEMENTVit C 2
0.1ml liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (0.04 mg/ml) Vitamin C and incubated at 37o C for 30 minutes.
DIETARY_SUPPLEMENTVit C 3
0.1ml liquefied semen was mixed with 0.1ml Ham's F10 medium supplemented with (0.06 mg/ml) Vitamin C and incubated at 37o C for 30 minutes.
Sponsors
Hawaa Fertility Center
Zagazig University
Benha University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Outcomes Assessor)
Intervention model description
control, vit E, vit C, Yeast
Eligibility
Sex/Gender
MALE
Age
22 Years to 35 Years
Healthy volunteers
No
Inclusion criteria
* must have been married for 1 year before inclusion into the study and were unable to achieve pregnancy * must have not received an antibiotic treatment for the last 4 weeks prior to sampling * showed oxidative stress- mediated asthenozoospermia in their semen
Exclusion criteria
* Severe male Factor
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| total motile sperm | 30 minutes | Number of motile sperms in prepared semen sample per high power field (HPF) |
| progressive motile sperm | 30 minutes | Number of progressively-motile sperms in prepared semen sample per HPF |
| vitality | 30 minutes | Number of Vital sperms in prepared semen sample per HPF |
| oxidative stress | 30 minutes | level of oxidative stress (OS) By Oxisperm Kit |
Countries
Egypt
Outcome results
None listed