Ascitic Fluid Microbiome in Liver Cirrhosis

Status

Withdrawn

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02813590

Enrollment

Registered

2016-06-27

Start date

2016-06-30

Completion date

2017-09-30

Last updated

2024-03-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Ascitic Fluid, Microbiota, Microbiota, Polymerase Chain Reaction, Sequence Analysis

Brief summary

Ascitic fluid microbiome is going to be investigated in this study by using internal transcripted spacer (ITS) and 16S ribosomal RNA (rRNA) polymerase chain reaction (PCR) and sequencing as conventional culture methods are lacking sensitivity regarding diagnosis of spontaneous bacterial peritonitis (SBP). All patients with routinely performed ascitic fluid puncture and underlying liver cirrhosis are included in this study. Next generation sequencing (NGS) in ascitic fluid, skin swaps from puncture side and stool samples as well as conventional culture methods are performed to investigate the difference in the microbiome between patients with and without SBP.

Interventions

OTHERAscitic fluid puncture

OTHERSkin swaps from puncture side

OTHERStool samples

Study design

Observational model

COHORT

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Inclusion criteria

* Underlying decompensated liver cirrhosis * routinely performed ascitic fluid puncture * informed consent

Exclusion criteria

Design outcomes

Primary

Measure	Time frame
Next generation sequencing results from ascitic fluid samples	1 year

Countries

Austria

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026