Staphylococcal Infection
Conditions
Keywords
biofilm, staphylokinase, Staphylococcus aureus, microarrays, bone binding sialoprotein, bone and joint infection, blood group
Brief summary
The purpose of this study is to identify bacterial and/or clinical features involved in the pathogenesis of Staphylococcus aureus implant-associated infections (IAI). Materials & methods: In total, 57 IAI S. aureus and 31 nasal carriage (NC) S. aureus isolates were studied. Staphylococcus aureus genetic background was obtained by microarray analysis. Multilocus sequence typing was performed to determine clonal complexes (CC). Biofilm production was investigated by resazurin and crystal violet methods
Interventions
OTHERIAI infection
OTHERStaphylococcus aureus nasal carriage infection
Sponsors
Nantes University Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* infected patients with S. Aureus IAI infection * noninfected patients screened for S. aureus nasal colonization at the orthopedic consultation ward
Exclusion criteria
* minor
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| biofilm production measurement in the two groups | september 2012 | The primary aim of this study is to identify phenotypic (biofilm production) markers in two different groups of S. aureus strains isolated from patients with IAI or screened from nasal carrier in noninfected patients undergoing orthopedic surgery. By using resazurin method, IAI and NC nasal carriage isolates were divided into three groups. Isolates were classified as strong, moderate and weak biofilm producers. |
| Clonal complexes distribution | september 2012 | The primary aim of this study is to identify genotypic markers in two different groups of S. aureus strains isolated from patients with IAI or screened from nasal carrier in noninfected patients undergoing orthopedic surgery. IAI and NC isolates genetic background was determined in order to compare the prevalence of individual virulence factor genes. All genes provided by the Alere StaphyType DNA microarray were studied. |
| IAI and NC nasal carriage isolates genetic background | september 2012 | IAI and NC isolates genetic background was determined in order to compare the prevalence of individual virulence factor genes. All genes provided by the Alere StaphyType DNA microarray were studied |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| biofilm production correlation with genotype | december 2013 | quantile-quantile (QQ)-plot analysis |
| Correlation between Clonal Complexes distribution , genotype and clinical parameters | december 2013 | In order to determine whether clusters of S. aureus could be associated with clinical characteristics of the patients, search of association between CCs and clinical data was performed |
| Association of ABO group phenotype and IAI S. aureus genotype | december 2013 | QQ-plots analysis |
Countries
France
Outcome results
None listed