High-intensity Interval Training Enhances Mobilization/Functionality of Endothelial Progenitor Cells and Depressed Shedding of Vascular Endothelial Cells Undergoing Hypoxia

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02802462

Enrollment

Registered

2016-06-16

Start date

2012-12-31

Completion date

2013-12-31

Last updated

2016-06-16

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Hypoxia

Keywords

endothelial progenitor cell, hemodynamic, Exercise, HIIT

Brief summary

Exercise training improves endothelium-dependent vasodilation, whereas hypoxic stress causes vascular endothelial dysfunction. Monocyte- derived endothelial progenitor cells (Mon-EPCs) contribute to vascular repair process by differentiating into endothelial cells. This study investigates how high-intensity interval (HIT) and moderate intensity-continuous (MCT) exercise training affect circulating Mon-EPC levels and EPC functionality under hypoxic condition. Sixty healthy sedentary males were randomized to engage either HIT (3-minute intervals at 40% and 80%VO2max , n=20) or MCT (sustained 60%VO2max , n=20) for 30 minutes/day, 5 days/week for 6 weeks, or to a control group that did not received exercise intervention (n=20). Mon-EPC characteristics and EPC functionality under hypoxic exercise (HE, 100W under 12%O 2 ) were determined before and after various interventions.

Interventions

BEHAVIORALHIT

3-minute intervals at 40% and 80%VO 2max

BEHAVIORALMCT

sustained 60%VO 2max

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

HEALTH_SERVICES_RESEARCH

Masking

NONE

Eligibility

Sex/Gender

MALE

Age

20 Years to 28 Years

Healthy volunteers

Yes

Inclusion criteria

* Sedentary life * Age 20\ 28

Exclusion criteria

* Smokers * Users of medication/vitamins * Any cardiopulmonary/hematological risk * Regular exercise habits at least 1 year * Exposed to high altitudes (\>3000 m) for at least 1 year

Design outcomes

Primary

Measure	Time frame	Description
Circulating progenitor cells conten	6 weeks	by flow cytometry before and after rehabilitation circulating progenitor cells conten before and after hypoxic exercise test

Secondary

Measure	Time frame
Cardiopulmonary fitness	6 weeks

Other

Measure	Time frame	Description
Cardiac hemodynamic measurement	6 weeks	evaluate cardiac hemodynamic response to exercise by noninvasive continuous cardiac output monitoring system
Cerebral hemodynamic measurement	6 weeks	Two pairs of near infrared probes were attached to each subject to monitor the absorption of near infrared light across left frontal cortex during graded exercise test
Muscular hemodynamic measurement	6 weeks	Two pairs of near infrared probes were attached to each subject to monitor the absorption of near infrared light across left vastus lateralis muscle during graded exercise test

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026