Healthy
Conditions
Keywords
exercise, performance, recovery, muscle, protein
Brief summary
Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA). Our recent study using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 30% higher than the recommended protein intake. The present study will investigate the impact of protein sufficiency on protein metabolism and performance during intensified training periods as a means to further our understanding of the nutritional requirements for the endurance athlete.
Interventions
DIETARY_SUPPLEMENTNormal
test drink which contains low amount of amino acids (0.14 g/kg/day)
DIETARY_SUPPLEMENTModerate
test drink which contains moderate amount of amino acids (0.40 g/kg/day)
DIETARY_SUPPLEMENTHigh
test drink which contains high amount of amino acids (1.03 g/kg/day)
OTHERControlled-diet
the diet containing 0.8 g protein /kg/day
Sponsors
Ajinomoto Co., Inc.
University of Toronto
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy, male, endurance-trained participants who have run regularly more than 45 km or 4.5 hours/week * Participants who are categorized at least very good based on a study by Shvartz & Reibold \[40\], in which peak rate of oxygen consumption(VO2 Peak) is used as an index. (i.e. the participants whose VO2peak is ≥57 ml/kg/min (18-24 y), ≥54 ml/kg/min (25-29 y), ≥52 ml/kg/min (30-34 y), ≥49 ml/kg/min (35-39 y), ≥47 ml/kg/min (40-44 y), ≥44 ml/kg/min (45-50 y) according to his age, * Participants who can cover 10 km in less than 60 min after the VO2peak test and 5 km Time trial on session 2. * Participants will be 18-50 years old. * Participants are willing to abide by the compliance rules of this study
Exclusion criteria
* Inability to meet health and physical activity guidelines according to the The Physical Activity Readiness Questionnaire for everyone. * Female * Inability to adhere to any of the compliance rules judged by principal investigator or medical doctor * Regular tobacco use * Illicit drug use (e.g. growth hormone, testosterone, etc.)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in 24h whole body protein balance | Difference between day 1 and day 4 whole body protein balance | Protein balance (mg/kg/d; protein synthesis - protein breakdown) will be measured using \[15N\]Glycine method on day 1 and day 4 to determine the change over the intensified training period to compare between protein levels |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change from baseline physical performance (5 km time trial) 5days after the beginning of training | 5days after the beginning of training | Before and after 4 days of intensified training period, Duration (seconds) for completing 5-km running on treadmill will be measured. |
| change from baseline Physical performance (muscle strength) 5days after the beginning of training | 5days after the beginning of training | Maximal strength will be assessed by a maximal isometric voluntary contraction (MVC), which will be tested for the knee-extensors using a custom-made knee-extension apparatus after 4 days intensified training. |
| change from baseline Physical Performance (muscle power) 5days after the beginning of training | 5days after the beginning of training | Peak lower body power will be measured by force platform after 4days-intensified training. |
Other
| Measure | Time frame | Description |
|---|---|---|
| Creatine Phosphate Kinase activity on day 5 | 5days after the beginning of training | Muscle damage markers (blood Creatine Phosphate Kinase activity, IU/L) will be compared between the experimental arms 5days after the beginning of training |
| amino acid concentration on day 5 | 5days after the beginning of training | amino acid concentration (mmol/mL) will be compared between the experimental arms 5days after the beginning of training |
| Myoglobin concentration on day 5 | 5days after the beginning of training | Muscle damage markers (blood Mb Concentration, mg/mL) will be compared between the experimental arms 5days after the beginning of training |
| IL-6 concentration on day 5 | 5days after the beginning of training | inflammatory markers (IL-6 concentration, pg/ml) will be compared between the experimental arms 5days after the beginning of training |
| CRP concentration on day 5 | 5days after the beginning of training | inflammatory markers (CRP concentration, mg/L) will be compared between the experimental arms 5days after the beginning of training |
Countries
Canada
Outcome results
None listed