Arterial Hypertension, CAD
Conditions
Keywords
arterial hypertension, microparticle, endothelial function
Brief summary
Membrane microparticles are submicron fragments of membrane vesicles shed from various cell types. Circulating endothelial microparticles have been proposed as markers of endothelial injury. However, which mechanical forces contribute to their release is not clear.
Detailed description
In a first series subjects (50% hypertensives) with and without arterial hypertension and no Coronary Artery Disease (CAD) (n=50) will be recruited. MP subpopulations will be discriminated by flow cytometry according to the expression of established surface antigens including CD31+/41-, CD144+, and CD62e+. Besides office and ambulatory 24h blood pressure measurements, pulse wave analysis will be performed to determine central blood pressure, augmentation index (AIX), and pulse wave velocity. Endothelial function (Flow-mediated dilation, FMD), arterial pulsatile stretch (fractional diameter changes, FDC), and wall-shear-stress (WSS) will be measured in the same segment of the brachial artery (BA) by ultrasound. In a second series, the investigators will take measurements in subjects with hypertensive crises (SBP\>180 mmHg) (n=20) before and after 4h and normalization of arterial BP by urapidil. In a third series, the investigators will take measurement in subjects with stable CAD (n=10) before and after transfemoral coronary diagnostic angiography.
Interventions
None listed
Sponsors
Klinik für Kardiologie, Pneumologie und Angiologie
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
MALE
Age
50 Years to 80 Years
Healthy volunteers
No
Inclusion criteria
* male subjects with or without arterial hypertension, hypertensive crises, stable CAD * written informed consent
Exclusion criteria
* subjects with manifest peripheral artery, or cerebrovascular disease, acute inflammation (CRP\>0.6 mg/dl), malignancies, arrhythmias
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| endothelial function | Baseline | endothelial function measured by flow-mediated dilation (FMD) for Subgroup 1 and 2 |
| Changes in endothelial function | Baseline and after 4 hours | endothelial function measured by flow-mediated dilation (FMD) for Subgroup 3 |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Changes Microparticle | Baseline and 4 hours after procedure | Microparticles (CD31+/41-, CD144+, and CD62e+) will be discriminated by flow cytometry for Subgroup 4 before and after transfemoral coronary diagnostic angiography |
| Wall Shear Stress | Baseline | measured by ultrasound in brachial artery for Subgroup 1 and 2 |
| Changes in Wall Shear Stress | Baseline and 4 hours | measured by ultrasound in brachial artery for Subgroup 3 |
| changes in fractional diameter (FDC) | Baseline | measured by ultrasound in brachial artery for Subgroup 1 and 2 |
| Microparticle | Baseline | Microparticles (CD31+/41-, CD144+, and CD62e+) will be discriminated by flow cytometry for Subgroup 1 and 2 |
| pulse wave velocity | Baseline | measured for Subgroup 1 and 2 |
| Changes in pulse wave velocity | Baseline and after 4 hours | measured for Subgroup 3 |
| augmentation index | baseline | measured for Subgroup 1 and 2 |
| Changes in augmentation index | Baseline and after 4 hours | measured for Subgroup 3 |
| Changes in fractional diameter (FDC) | Baseline and 4 hours after procedure | measured by ultrasound in brachial artery for Subgroup 4 before and after transfemoral coronary diagnostic angiography |
| Changes in Microparticle | Baseline and after 4 hours | Microparticles (CD31+/41-, CD144+, and CD62e+) will be discriminated by flow cytometry for Subgroup 3 |
Countries
Germany
Outcome results
None listed