Human Chorionic Gonadotropin (hCG) Stimulation, Klinefelter Syndrome, Steroidogenesis
Conditions
Brief summary
The study design included six visits. During the first visit (visit 0), the subjects underwent physical examination(height, weight, body mass index (BMI), arm span, and upper segment measurement) and testicular ultrasound (US) for the calculation of testicular volume. At 0800 h of day 0, all subjects provided a basal blood sample immediately followed by a single intramuscular injection of hCG of 5000 IU. Further five visits were performed each of five following consecutive days after the hCG injection. A blood sample was taken at each visit after an overnight fast
Detailed description
Blood samples were centrifuged at 2955 g for 15 min. Sera were transferred into plain polypropylene tubes and stored at -20°C until assayed. P, 17OHP, AS, DHEA, and TS were determined by LC-MS/MS at the laboratory of the Centre for Applied Biomedical Research of the S. Orsola-Malpighi Hospital, Alma Mater Studiorum, University of Bologna, Bologna, Italy.
Interventions
DRUGhuman chorionic gonadotropin
At 08 AM of day 0, all subjects underwent to a single intramuscular injection of hCG of 5000 IU
Sponsors
Azienda USL Modena
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Klinefelter syndrome diagnosis at kariotype evaluation for the study group * Healthy age-matched men in the control group
Exclusion criteria
* previous or ongoing androgen therapy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Testosterone | visit 1 (Day 1) | Using mass spectrometry |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Testicular volume | visit 0 (Baseline) | testicular volume at ultrasonography |
| Steroids | Visit 0 (Baseline) | Steroidogenesis evaluated through mass spectrometry |
Countries
Italy
Outcome results
None listed