Sub-optimal Culture Conditions
Conditions
Brief summary
Culture media used to support development of zygotes to the blastocyst stage is based on two basic philosophies: a single step medium let-the-embryo-choose or a sequential medium back-to-nature. For single step media, there are two types of protocol can be used for the culture of human preimplantation embryos: 1. Interrupted single medium culture, in which the medium is renewed on the Day 3 of embryo culture 2. Uninterrupted single embryo culture, in which the same single medium is used throughout the 5-6 days of culture with no replenishment or replacement on Day 3. Investigators aim to compare the blastocyst development and clinical outcome between the two types of protocols of a single medium (with or without renewal on Day 3)
Interventions
PROCEDURECulture media renwal
Sponsors
Dar AlMaraa Center
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Masking
DOUBLE (Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
FEMALE
Age
19 Years to 39 Years
Healthy volunteers
No
Inclusion criteria
* female partners were under the age of 39 years * Intracytoplasmic sperm injection (ICSI) was performed using fresh ejaculates
Exclusion criteria
* Day 3 embryo transfer cycles
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Clinical pregnancy rate | 4 weeks post embryo transfer | diagnosed by the presence of a heartbeat in the gestational sac visualized by ultrasound |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Implantation rate | 4-6 weeks post embryo transfer | the number of intrauterine gestational sacs over the total number of embryos transferred |
| Blastocyst formation rate | 5-6 days post intracytoplasmic sperm injection | — |
Countries
Egypt
Contacts
Primary ContactYasmin Magdi, M.Sc
Backup ContactAhmed El-Damen, M.Sc
Outcome results
None listed