Biofilm, Dental Plaque
Conditions
Keywords
pyrosequencing, 16S DNA gene, Confocal Laser Scanning Microscope, bacterial viability, bacterial diversity
Brief summary
The purpose of this study is to determine in situ the bacterial viability, thickness and bacterial diversity of a biofilm formed on different substrates in comparison to a naturally tooth-formed oral biofilm. In addition, it will be evaluated whether the restraint of oral hygiene measures may influence the development of the PL-Biofilm.
Detailed description
* To calculate an a priori sample size, the following statistical criteria were established: an effect size of 0.35, an alpha error of 0.05 and a statistical power of 87%. Assuming these criteria and using the repeated measures ANOVA test, a sample size of 15 subjects was required. * Bacterial viability and thickness will be analyse by Confocal Laser Scanning Microscope and Live/Dead BackLight staining solution. * Bacterial diversity will be analyse by Pyrosequencing the 16S DNAr gene.
Interventions
OTHERToothbrushing
Normal toothbrushing without toothpaste or antiseptic mouthwash.
Sponsors
University of Santiago de Compostela
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
20 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* systemically healthy * good oral health status
Exclusion criteria
* smoker or former smoker, * presence of dental prostheses or orthodontic devices, * antibiotic treatment or routine use of oral antiseptics in the previous 3 months, * presence of any systemic disease that could alter the production or composition of saliva
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Bacterial viability | Bacterial viability measured in proportion of viable/no viable bacteria after 48 hours of exposition to oral cavity | It will be measured by analysis of images taken by Confocal laser Scanning Microscope |
| Bacterial diversity | Bacterial diversity measured by microbial estimators of abundance and richness after 48 hours of exposition to oral cavity | It will be measured by 16S rDNA gene pyrosequencing and posterior bioinformatic analysis. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Biofilm thickness | Oral biofilm thickness measured by analysis of sectional images after 48 hours of exposition to oral cavity | It will be measured by analysing sectional images taken by Confocal Laser Scanning Microscope. |
Countries
Spain
Contacts
Primary ContactInmaculada Tomás Carmona, S Lecturer
Outcome results
None listed