Bioavailability
Conditions
Keywords
Mediterranean diet, indoleamine, nutraceuticals, nutrition, oxidative stress
Brief summary
Melatonin (MLT) is a bioactive compound in red wine. The investigators will investigate the serum and salivary concentrations and antioxidant power of MLT after the intake of a glass of red wine. Twelve healthy volunteers were recruited to receive both 125 mL of red wine rich in MLT (10 ng mL-1) and without MLT (placebo), via a randomized, cross-over, and double-blind design. Serum and salivary MLT will be measured by liquid chromatography coupled to mass spectrometry (LC-MS) and enzyme-linked immunosorbent (ELISA), respectively. The ferric ion reducing antioxidant power (FRAP) will be analyzed.
Detailed description
Melatonin (MLT) is a bioactive compound in red wine. No information is currently available on serum and salivary concentrations of MLT after drinking a glass of red wine, containing this indoleamine. Hence, in this study, the investigators will investigate the levels of MLT, in serum and saliva, soon after the intake of the MLT-rich red wine, and, correspondingly, they will verify the antioxidant power of the same biological fluids. This will be a randomized, crossover, double-blind, placebo-controlled study with two treatment arms. Twelve healthy volunteers will be recruited to receive both 125 mL of red wine rich in MLT (10 ng mL-1) and without MLT (placebo), via a randomized, cross-over, and double-blind design. Serum and salivary MLT will be measured by liquid chromatography coupled to mass spectrometry (LC-MS) and enzyme-linked immunosorbent (ELISA), respectively. The ferric ion reducing antioxidant power (FRAP) will be also analyzed. For serum analyses, samples will be collected at 0, 30, 60 and 90 min. For saliva analyses, samples will be collected at 0, 45 and 120 min.
Interventions
OTHERRed wine with melatonin
Twelve healthy volunteers will be recruited to receive both 125 mL of red wine rich in MLT (10 ng mL-1)
OTHERRed wine without melatonin
Twelve healthy volunteers will be recruited to receive both 125 mL of red wine without MLT
Sponsors
University of Milan
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
20 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* to be healthy * to have normal weight (BMI 18.5-25.0) * to voluntarily accept to participate to the study.
Exclusion criteria
* pregnancy and lactation for women; * supplementation with MLT and/or any other dietary supplement (vitamins, antioxidants, botanicals, phytochemicals) * systemic and chronic-degenerative diseases * abnormal hematological parameters * heavy smoking and alcohol drinking * high-intensity physical activity * under pharmacological treatment
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Serum and salivary melatonin after red wine intake (with or without MLT) | Time-course of the serum melatonin levels in healthy volunteer from 0 min to 90 min after administration of red wine. Time-course of the salivary melatonin levels in healthy volunteer from 0 min to 120 min after administration of red wine. | LC-MS analyses for serum and ELISA analyses for saliva |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Antioxidant power of saliva and serum after red wine intake (with or without MLT) | Time-course of the serum antioxidant power in healthy volunteer from 0 min to 90 min after administration of red wine. Time-course of the salivary antioxidant power in healthy volunteer from 0 min to 120 min after administration of red wine. | FRAP analyses |
Other
| Measure | Time frame | Description |
|---|---|---|
| Adverse effects | Acute side effects during the trial recorded at each experimental day each experimental day, up to 1 day after | Questionnaire |
Countries
Italy
Outcome results
None listed