Biomarkers of Air Pollution Exposure
Conditions
Keywords
Environmental Pollution, Fine particles, Biomarkers, Elderly
Brief summary
Nowadays, an increase of inflammatory chronic diseases and/or tumors incidence has been reported in part due to the rising in life expectancy. For instance, exposure to air pollution, and in particularly to fine particles has been classified by the International Agency for Research on Cancer (IARC) as a risk factor for cancer, being the elderly population particularly sensitive. However, no validated biomarkers have been identified to assess the exposure to fine particles maybe correlated to cancerogenesis. AEROTOX-2 is a prospective pilot study on the ex vivo effects of the atmospheric pollution exposure. The study aims to identity new biomarkers after an exposure of leukocytes to doses of fine particles. Secondly, the study aims to analyze, according to age, the leukocytes response to the urban pollution exposure.
Detailed description
To estimate the influence of the atmospheric pollution on the whole adult population, subjects will be recruited in a wide range of age (20 - 80 years). Samples of atmospheric particles will be collected in Dunkerque (North of France), one of the most important industrial areas in France. This city is influenced by car traffic, urban and industrial activities, and the wide sea traffic of the North Sea. Therefore, Dunkerque is a reference site to study the atmospheric pollution and its impact on health. These markers will be searched in association with the different mechanisms involved in the toxicity of the fine particles such as: inflammatory response, genotoxicity, metabolic activity and epigenetic changes after exposure of leukocytes isolated from patients to doses of fine particles. The study of the correlation between biological observations and conditions of exposure to air particles will bring answers about the impact of the environmental pollution on the health of vulnerable populations.
Interventions
PROCEDUREBlood sampling
Sponsors
Lille Catholic University
Universite du Littoral Cote d'Opale
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
20 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
* Age between 20-30 years, 45-55 years or 70-80 years * Signature of an informed consent * Non-smoker or ex-smoker for more than 10 years * Homogeneous repartition between men and women * Social insurance affiliation * Understanding or being able to speak French
Exclusion criteria
* Pregnant or breast feeding woman * Treatment with parenteral corticoids in the 30 days prior to inclusion visit, treatment with immunosuppressant drugs, radiotherapy, chemotherapy * Occupational exposure (metallurgy, petrochemistry, house painter) for more than 10 years and having ceased all activities in these areas for less than 10 years ago.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Level of expression of blood biomarkers of air pollution exposure | through the study completion, an average of 48 months | These markers will be searched in association with the different mechanisms involved in the toxicity of the fine particles such as: inflammatory response, genotoxicity, metabolic activity and epigenetic changes after exposure of leukocytes to doses of fine particles. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Total amount of different populations of peripheral blood leucocytes | through the study completion, an average of 48 months | After exposure of leukocytes to doses of fine particles. |
| Measure of the expression of genes involved in metabolism by quantitative polymerase chain reaction (qPCR) | through the study completion, an average of 48 months | After exposure of leukocytes to doses of fine particles. |
| Measure of the expression of genes involved in metabolism by TaqMan Gene Expression Assays | through the study completion, an average of 48 months | After exposure of leukocytes to doses of fine particles. |
| Detection of genotoxicity by identification of DNA double-strand breaks and DNA adducts | through the study completion, an average of 48 months | — |
| Detection of epigenetic modifications | through the study completion, an average of 48 months | After exposure of leukocytes to doses of fine particles. In order to measure the expression of hundreds of miRNAs within a given sample a microarray analysis technology will be performed using the TaqMan® Low Density Array Human MicroRNA. |
| Correlation between age and markers of inflammation | through the study completion, an average of 48 months | After exposure of leukocytes to doses of fine particles. |
| Correlation between age and markers of metabolic activity | through the study completion, an average of 48 months | After exposure of leukocytes to doses of fine particles. |
| Correlation between age and markers genotoxicity | through the study completion, an average of 48 months | After exposure of leukocytes to doses of fine particles. |
| Correlation between age and markers of epigenetic modifications | through the study completion, an average of 48 months | After exposure of leukocytes to doses of fine particles. |
Countries
France
Contacts
PRINCIPAL_INVESTIGATORGosset Pierre, MD
Lille Catholic University
STUDY_CHAIRSylvain Billet
UCEIV
Outcome results
None listed