Antibacterial Effectiveness of Two Root Canal Irrigants

Antibacterial Effectiveness of Two Root Canal Irrigants in Root-filled Teeth With Infection: A Randomized Clinical Trial

Status

Completed

Phases

Phase 2

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02747082

Enrollment

Registered

2016-04-21

Start date

2012-01-31

Completion date

2013-01-31

Last updated

2016-04-21

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Periapical Periodontitis

Keywords

endodontic retreatment, sodium hypochlorite, chlorhexidine, calcium hydroxide, Retreatment

Brief summary

The aim of this randomized clinical trial is to compare the antibacterial effects of 1% sodium hypochlorite (NaOCl) and 2% chlorhexidine gluconate (CHX) during retreatment of teeth with apical periodontitis (AP).

Detailed description

Root canal-treated teeth with AP were randomly distributed in two groups according to the irrigant used. After removal of the previous filling material, bacteriological samples were taken from the canals before (S1) and after chemomechanical preparation using either NaOCl or CHX (S2), and after calcium hydroxide medication for 2-3 weeks (S3). 16S rRNA gene-based real-time qPCR was performed to detect and quantify total bacteria, Streptococcus species and Enterococcus faecalis.

Interventions

PROCEDURERetreatment of root-filled teeth with infection

Root-filled teeth with infection are being treated with either 1% NaOCl or 2% CHX as disinfection irrigation solution.

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

TREATMENT

Masking

SINGLE (Subject)

Eligibility

Sex/Gender

ALL

Age

20 Years to 95 Years

Healthy volunteers

Inclusion criteria

* Clinical diagnosis of apical periodontitis in a root-filled tooth assessed by radiography.

Exclusion criteria

* Presence of gross carious lesions in the diagnosed tooth, * Presence of fractures involving the periodontium of the diagnosed tooth, * Presence of periodontal pockets more than 4 mm deep in the diagnosed tooth, * Diagnosis of diabetes, * Diagnosis of HIV infection, * Other immunocompromising disease, * Antibiotic therapy within the previous 3 months.

Design outcomes

Primary

Measure	Time frame
Periapical radiolucency (PAI- and Friedman-scores)	12 months

Secondary

Measure	Time frame
Bacterial load assessed by qPCR	21 days
Pyrosequencing of species found before and after the intervention	21 days

Countries

Norway

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026