Human Microbiome
Conditions
Keywords
Gluten-Free diet, Healthy individuals
Brief summary
Changes in the gut microbiomes of healthy volunteers who followed a gluten-free diet (GFD) for four weeks were studied. Per participant, nine stool samples were collected: one at baseline, four during the GFD period, and four when they returned to their habitual diet. Microbiome profiles were determined using 16S rRNA sequencing and the samples were next processed for taxonomic and imputed functional composition. Additionally, participants kept a 3-day food diary and six gut health-related biomarkers were measured.
Interventions
OTHERDietary intervention
Sponsors
University Medical Center Groningen
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
16 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* healthy participants aged \>=16
Exclusion criteria
* Known gastrointestinal disorder * Known food intolerance
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Changes in gut microbiota composition upon gluten-free diet intervention | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
Secondary
| Measure | Time frame |
|---|---|
| Changes in gut associated biomarker levels: plasma cytokines: IL-6 | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
| Changes in gut associated biomarker levels: fecal calprotectin | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
| Changes in gut associated biomarker levels: plasma cytokines: IL-1beta | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
| Changes in gut associated biomarker levels: fecal human beta defensin 2 | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
| Changes in gut associated biomarker levels: fecal chromogranin A | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
| Changes in gut associated biomarker levels: fecal short chain fatty acids: propionate | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
| Changes in gut associated biomarker levels: plasma citrulline | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
| Changes in gut associated biomarker levels: fecal short chain fatty acids: caproate | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
| Changes in gut associated biomarker levels: fecal short chain fatty acids: butyrate | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
| Changes in gut associated biomarker levels: fecal short chain fatty acids: valerate | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
| Changes in gut associated biomarker levels: plasma cytokines: IL-8 | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
| Changes in gut associated biomarker levels: plasma cytokines: IL-10 | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
| Changes in gut associated biomarker levels: plasma cytokines: IL-12 | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
| Changes in gut associated biomarker levels: plasma cytokines: TNFalpha | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
| Changes in gut associated biomarker levels: fecal short chain fatty acids: acetate | baseline, 4 weeks gluten-free diet and 4 weeks habitual diet |
Countries
Netherlands
Outcome results
None listed