Mitochondrial Energy Production in ART (Assisted Reproductive Technology )

Impact on Mitochondrial Energy Production in Different Patient Populations in Women Undergoing IVF-ICSI (in Vitro Fertilization-intracytoplasmic Sperm Injection)

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02727400

Acronym

Mitenergy

Enrollment

Registered

2016-04-04

Start date

2017-04-01

Completion date

2018-04-16

Last updated

2018-04-17

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Female Infertility Due to Advanced Maternal Age

Brief summary

Oocyte maturation requires a lot of energy, which is provided by the mitochondria via the synthesis of ATP. The majority of patients with advanced maternal age (AMA) have poor egg quality. One of the reasons depends on oxidative phosphorylation of Pyruvate to undergo maturation; on the contrary, the cells of the cumulus (CC) show great activity glycolytic so that these cells are able to provide ATP(adenosine triphosphate and energy substrates to the oocyte. The goal of this work is to analyze the mitochondrial function and energy production in oocyte donors compared with a group of women of advanced maternal age subject to a same ovarian stimulation Protocol

Interventions

None listed

Study design

Observational model

CASE_CONTROL

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

FEMALE

Age

18 Years to 46 Years

Healthy volunteers

Yes

Inclusion criteria

* Controls: Women between 18-35 years old who are oocyte donors and fulfill the requirements of the our program of oocyte donation according to the Spanish Law of Human Reproduction * Cases: infertile women undergoing IVF due to advanced maternal age (\>38 years)

Exclusion criteria

For controls: Women with antral follicle count \>20 or \<6 follicle per ovary or with pathologies which, in the opinion of the investigator, may interfere with the treatment of ovarian stimulation * Severe hypersensitivity to drugs of similar structure * In the case of associated pathologies which can be considered as

Design outcomes

Primary

Measure	Time frame	Description
Adenosine Triphosphate (ATP) production (nmol)	15 days	ATP levels with the ATP assay kit (ab113849) Succinate dehydrogenase (SDH) activity assay kit (BioVision, USA) Mitochondrial Complex IV by colorimetric determination of oxidation-reduction processes of Cytochrome C

Countries

Spain

Outcome results