The Effect of Anesthetics on Oxidative Stress and Apoptosis Status in Children

The Effect of Sevoflurane and Propofol on Oxidative Stress and Apoptosis Status in Children Undergoing Hypospadias Repair Surgery

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02711280

Enrollment

Registered

2016-03-17

Start date

2015-09-30

Completion date

2016-06-30

Last updated

2016-10-12

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Anesthesia, General

Brief summary

The purpose of this study is to determine whether Propofol and Sevoflurane affect the oxidative stress and apoptosis status in children undergoing hypospadias repair surgery.

Interventions

DRUGSevoflurane

DRUGPropofol

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

BASIC_SCIENCE

Masking

SINGLE (Subject)

Eligibility

Sex/Gender

MALE

Age

1 Years to 3 Years

Healthy volunteers

Inclusion criteria

* American Society of Anesthesiology (ASA) I-II, undergoing selective surgery for hypospadias repair.

Exclusion criteria

* a history of developmental delay or mental retardation, which could make observational pain intensity assessment outside the norm; * a history of central nervous system diseases; * a known or suspected coagulopathy; * a known allergy to any of the study drugs; * abnormalities of the sacrum and any signs of infection at the site of the proposed caudal block.

Design outcomes

Primary

Measure	Time frame	Description
the change of blood glutathione peroxidase(GPx) level in perioperative period	immediately after anesthetic induction, 2h and 3 days after operation	—
the change of blood-separated erythrocytes superoxide dismutase (SOD) activities in perioperative period	immediately after anesthetic induction, 2h and 3 days after operation	the blood sample was separated and erythrocytes was obtained, superoxide dismutase activity was measured using a commercially available assay-Ransod kit (Randox Laboratories, UK).
the change of blood-separated erythrocytes catalase (CAT) activities in perioperative period	immediately after anesthetic induction, 2h and 3 days after operation	the blood sample was separated and erythrocytes was obtained, catalase activity was measured using the OxiSelect catalase activity assay (Cell Biolabs, San Diego, CA, USA)
the change of blood-separated lymphocytes caspase-3 mRNA level in perioperative period	immediately after anesthetic induction, 2h and 3 days after operation	Lymphocytes were separated from whole blood and freshly used for real-time PCR of caspase-3

Countries

China

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026