Analysis of Lipid Profiles in Samples Sperm and Seminal Plasma Under Artificial Insemination

Status

Withdrawn

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02704936

Enrollment

Registered

2016-03-10

Start date

2015-12-31

Completion date

2018-10-31

Last updated

2021-09-05

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Primary Infertility

Brief summary

Identify, by Lipidomics approaches, biochemical markers of fertility / infertility in sperm and seminal plasma linking the success or failure of the artificial insemination (IUI)

Detailed description

Analyze lipid profiles in samples of sperm and seminal plasma of three populations: 1. male sperm donors 2. men assigned to a program of assisted reproduction with normal sperm / slightly abnormal successfully in the IUI; 3. attached to a program of assisted reproduction with male semen Normal / slightly abnormal unsuccessfully in IUI Establish partnerships between the composition of fatty acids, especially fatty acids polyunsaturated (PUFA) of the omega-3 (w3) series of samples analyzed parameterssemen and results of the IA to identify biochemical markers of fertility. Assess markers found by checking the increase in the rate of success in the IA.

Interventions

None listed

Study design

Observational model

COHORT

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

FEMALE

Age

18 Years to 38 Years

Healthy volunteers

Yes

Inclusion criteria

Group A women donor group * Fallopian unobstructed Men donor group * Appear as sperm donor * Age between 18-35 years * Sexual abstinence 3-5 days

Exclusion criteria

Group A: * Uterine malformations (fibroids, adenomyosis, uterine malformations or acquired congenital) and fallopian tubes (tubal occlusion) * Obesity BMI (Body Mass Index)\> 30 kg / m2 * Presence of altered tubal toxic habits (smoking\> 15 cigarettes per day, alcohol or drug abuse). Inclusión /

Design outcomes

Primary

Measure	Time frame	Description
Identify, by Lipidomics approaches, biochemical markers of fertility / infertility sperm and seminal plasma linking the success or failure of the artificial insemination	18 months	Levels of docosahexaenoic acid (DHA) and acid eicosapentaenoic (EPA) in 42 semen samples: * With positive pregnancy in any of the first 4 treatments of artificial insemination. * Gestacion Negative in artificial insemination * Assigned to the program-donors donation

Secondary

Measure	Time frame	Description
Descriptive analysis of semen parameters versus DHA and EPA levels	18 months	Correlation analysis of seminal variables: sperm count, motility and morphology percent compared to the levels of DHA and EPA obtained. Assuming normal distribution continuous variables, ANOVA test apply. If the normality assumption failed an Kruskal-Wallis test was applied.

Countries

Spain

Outcome results