Changes in Alveolar Macrophage Function During Acute Lung Injury

Status

UNKNOWN

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02693912

Enrollment

Registered

2016-02-29

Start date

2016-09-30

Completion date

2018-08-31

Last updated

2016-02-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Acute Lung Injury

Keywords

efferocytosis, macrophage

Brief summary

In this study, the investigators aim to observe the change in alveolar macrophage function during acute lung injury.

Detailed description

Alveolar macrophages are tissue-resident or recruited cells with key functions in recognition of pathogens, initiation of host defensive ang protective inflammation,and in clearance of pathogens from the airways. However, macrophages are also responsible for efficient clearance of apoptotic neutrophils, termed efferocytosis, which is a prerequisite for the timely resolution of inflammation. In this study, the investigators aim to observe the change in alveolar macrophage function during acute lung injury.

Interventions

PROCEDUREBronchoscopy

Bronchoscopy will be implemented under sedation in both groups.

Study design

Observational model

CASE_CONTROL

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Yes

Inclusion criteria

* Patients in ICU with diagnosed ALI under mechanical ventilation

Exclusion criteria

* Patients with cardiac dysfunction, using steroid drugs or other drugs can that affect efferocytosis

Design outcomes

Primary

Measure	Time frame	Description
alveolar macrophages (AMs) phagocytic index	7 days after admission	AMs will be collected and cultured in vitro. Apoptotic neutrophils will then be cocultured with AMs for 1 hour. To measure phagocytosis of apoptotic neutrophils, AMs were further processed and phagocytosed apoptotic neutrophils were detected by confocal fluorescence microscopy as described below under Cell staining and confocal microscopy. The phagocytic index was calculated according to the following formula: phagocytic index = (total number of engulfed cells/total number of counted macrophages) × (number of macrophages containing engulfed cells/total number of counted macrophages) × 100.

Secondary

Measure	Time frame
30 days mortality	30 days after admission

Contacts

Primary ContactChunling Jiang

jiang_chunling@yahoo.com

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026