Cerebral Arterial Aneurysm
Conditions
Brief summary
The purpose of this study was to evaluate differences between propofol postconditioning and sevoflurane anesthesia in the intracranial aneurysm surgery about antioxidant effect.
Detailed description
intracranial aneurysm surgery may lead to regional ischemic reperfusion injury. Propofol, as an antioxidative phenol, has been demonstrated that mitigate the brain I/R in rats. As for sevoflurane, some investigation indicated that sevoflurane is able to reduce oxidative stress in cell(except neuronal cell lines ) and rodent models, but the antioxidant effect did not found in human minor incision surgeries, furthermore, oxidative stress was raised in the major surgeries including orthopedic surgeries, hysterectomy, cholecystectomy, and thoracotomy
Interventions
DRUGpropofol postconditioning
administrated Target Controlled Infusion(TCI) of propofol (Cp 1.2ug/ml) and decreased sevoflurane with a Bispectral index (BIS) value of 40-60 to maintain anesthesia after clamp removal
DRUGsevoflurane
0.5%-2% sevoflurane with BIS 40-60
Sponsors
The Third Central Clinical College of Tianjin Medical University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
DOUBLE (Subject, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* Clinical diagnosis intracranial aneurysm surgery , * American Society of Anesthesiologists (ASA)Ⅰ-Ⅱ.
Exclusion criteria
* severe hepatic and renal dysfunction * coagulation disorder * taking antioxidant perioperative period
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Evidences of Clinically Definite Oxidative Stress: Nucleoplasmic Bridges | before induction,clamping removal ,operation ending,1,3,7days post surgery | Evidences of clinically definite oxidative stress: nucleoplasmic bridges confirmed by Cytokinesis-block Micronucleus Test |
| Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | before induction,after clamping removal ,operation ending ,1,3,7days post surgery | Evidences of clinically definite oxidative stress :α- tocopherol,γ- tocopherol which was used to assess the antioxidant defense. |
| Evidences of Clinically Definite Oxidative Stress: Nuclear Buds | before induction,clamping removal ,operation ending,1,3,7days post surgery | Evidences of clinically definite oxidative stress:nuclear buds Confirmed by Cytokinesis-block Micronucleus Test |
| Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | before induction,after clamping removal ,operation ending ,1,3,7days post surgery | Evidences of clinically definite oxidative stress :Superoxide dismutase activity, Hydroxyl radical |
| Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA | before induction, after clamping removal,operation ending,1,3,7days post surgery | Evidences of clinically definite oxidative stress:8-isoprostane,as a reliable biomarkers of lipid peroxidation |
| Evidences of Clinically Definite Oxidative Stress:Micronuclei | before induction,clamping removal ,operation ending,1,3,7days post surgery | Evidences of clinically definite oxidative stress:micronuclei confirmed by Cytokinesis-block Micronucleus Test |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Montreal Cognitive Assessment (MoCA) | before induction,1,3,7days post surgery | A questionnaires is used to assess the cognitive function of patients in clinical,the total range was 0-30,and 27-30 were considered as normal value,\<27 were considered as recognitive dysfunction. |
| Mini Mental State Examination (MMSE) | before induction,1,3,7days post surgery | A questionnaires is used to assess the cognitive function of patients in clinical,the total range was 0-30,and 27-30 were considered as normal value,\<27 were considered as recognitive dysfunction. |
Participant flow
Recruitment details
From July 2014 to January 2016, patients who were undergoing intracranial aneurysm surgery at hospital and consistent with the criterion of American Society of Anesthesiologists (ASA)Ⅰ-Ⅱ,but without severe hepatic and renal dysfunction, coagulation disorder were recruited.
Participants by arm
| Arm | Count |
|---|---|
| Propofol Postconditioning 1.2ug/ml propofol Group propofol postconditioning was administrated TCI of propofol (Cp 1.2ug/ml) and decreased sevoflurane concentration with a BIS value of 40-60 to maintain anesthesia after clamp removal immediately. | 30 |
| Sevoflurane 0.5%-2% sevoflurane
sevoflurane: 0.5%-2% sevoflurane with BIS 40-60 | 30 |
| Total | 60 |
Baseline characteristics
| Characteristic | Propofol Postconditioning | Sevoflurane | Total |
|---|---|---|---|
| Age, Categorical <=18 years | 0 Participants | 0 Participants | 0 Participants |
| Age, Categorical >=65 years | 0 Participants | 0 Participants | 0 Participants |
| Age, Categorical Between 18 and 65 years | 30 Participants | 30 Participants | 60 Participants |
| Region of Enrollment China | 30 participants | 30 participants | 60 participants |
| Sex: Female, Male Female | 16 Participants | 17 Participants | 33 Participants |
| Sex: Female, Male Male | 14 Participants | 13 Participants | 27 Participants |
Adverse events
| Event type | EG000 affected / at risk | EG001 affected / at risk |
|---|---|---|
| deaths Total, all-cause mortality | — / — | — / — |
| other Total, other adverse events | 0 / 30 | 0 / 30 |
| serious Total, serious adverse events | 0 / 30 | 0 / 30 |
Outcome results
Primary
Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA
Evidences of clinically definite oxidative stress:8-isoprostane,as a reliable biomarkers of lipid peroxidation
Time frame: before induction, after clamping removal,operation ending,1,3,7days post surgery
| Arm | Measure | Group | Value (MEAN) | Dispersion |
|---|---|---|---|---|
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA | 8-isoprostane of operation ending | 1.54 pg/ml | Standard Deviation 0.16 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA | 8-isoprostane of 3days post surgery | 1.48 pg/ml | Standard Deviation 0.17 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA | 8-isoprostane of clamping removal | 1.19 pg/ml | Standard Deviation 0.15 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA | 8-isoprostane of 7days post surgery | 1.38 pg/ml | Standard Deviation 0.18 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA | 8-isoprostane of before induction | 1.15 pg/ml | Standard Deviation 0.14 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA | 8-isoprostane of 1days post surgery | 1.51 pg/ml | Standard Deviation 0.16 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA | 8-isoprostane of 7days post surgery | 1.49 pg/ml | Standard Deviation 0.19 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA | 8-isoprostane of clamping removal | 1.22 pg/ml | Standard Deviation 0.14 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA | 8-isoprostane of operation ending | 1.70 pg/ml | Standard Deviation 0.18 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA | 8-isoprostane of 1days post surgery | 1.64 pg/ml | Standard Deviation 0.21 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA | 8-isoprostane of 3days post surgery | 1.60 pg/ml | Standard Deviation 0.18 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA | 8-isoprostane of before induction | 1.18 pg/ml | Standard Deviation 0.14 |
Primary
Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit
Evidences of clinically definite oxidative stress :Superoxide dismutase activity, Hydroxyl radical
Time frame: before induction,after clamping removal ,operation ending ,1,3,7days post surgery
| Arm | Measure | Group | Value (MEAN) | Dispersion |
|---|---|---|---|---|
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Superoxide dismutase activity of before induction | 72.87 U/ml | Standard Deviation 23.15 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Superoxide dismutase activity of clamping removal | 67.10 U/ml | Standard Deviation 16.52 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Superoxide dismutase activity of operation ending | 66.74 U/ml | Standard Deviation 16.5 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Superoxide dismutase activity of 1days postsurgery | 66.80 U/ml | Standard Deviation 16.56 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Superoxide dismutase activity of 3days postsurgery | 69.27 U/ml | Standard Deviation 16.66 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Superoxide dismutase activity of 7days postsurgery | 69.02 U/ml | Standard Deviation 16.95 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Hydroxyl radical of before induction | 5.30 U/ml | Standard Deviation 6.46 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Hydroxyl radical of clamping removal | 15.80 U/ml | Standard Deviation 4.42 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Hydroxyl radical of operation ending | 12.54 U/ml | Standard Deviation 4.32 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Hydroxyl radical of 1days post surgery | 10.32 U/ml | Standard Deviation 4.61 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Hydroxyl radical of 3days post surgery | 8.05 U/ml | Standard Deviation 4.8 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Hydroxyl radical of 7days post surgery | 6.33 U/ml | Standard Deviation 5.57 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Hydroxyl radical of 3days post surgery | 12.46 U/ml | Standard Deviation 5.33 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Superoxide dismutase activity of before induction | 74.02 U/ml | Standard Deviation 21.27 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Hydroxyl radical of before induction | 6.60 U/ml | Standard Deviation 6.49 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Superoxide dismutase activity of clamping removal | 65.91 U/ml | Standard Deviation 16.07 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Hydroxyl radical of 1days post surgery | 13.65 U/ml | Standard Deviation 5.37 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Superoxide dismutase activity of operation ending | 51.58 U/ml | Standard Deviation 14.92 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Hydroxyl radical of clamping removal | 16.22 U/ml | Standard Deviation 5.23 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Superoxide dismutase activity of 1days postsurgery | 52.82 U/ml | Standard Deviation 14.93 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Hydroxyl radical of 7days post surgery | 10.32 U/ml | Standard Deviation 5.16 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Superoxide dismutase activity of 3days postsurgery | 57.35 U/ml | Standard Deviation 17.36 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Hydroxyl radical of operation ending | 15.29 U/ml | Standard Deviation 5.3 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by ELISA Kit | Superoxide dismutase activity of 7days postsurgery | 59.33 U/ml | Standard Deviation 17.3 |
Primary
Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography
Evidences of clinically definite oxidative stress :α- tocopherol,γ- tocopherol which was used to assess the antioxidant defense.
Time frame: before induction,after clamping removal ,operation ending ,1,3,7days post surgery
| Arm | Measure | Group | Value (MEAN) | Dispersion |
|---|---|---|---|---|
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | γ- tocopherol of 7days post surgery | 0.68 ug/ml | Standard Deviation 0.13 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | α- tocopherol of opertaion ending | 6.71 ug/ml | Standard Deviation 1.79 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | α- tocopherol of 1days post surgery | 8.97 ug/ml | Standard Deviation 1.87 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | α- tocopherol of 3days post surgery | 10.02 ug/ml | Standard Deviation 2.06 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | α- tocopherol of 7days post surgery | 11.54 ug/ml | Standard Deviation 1.84 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | γ- tocopherol of before induction | 0.52 ug/ml | Standard Deviation 0.08 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | γ- tocopherol of clamping removal | 0.59 ug/ml | Standard Deviation 0.09 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | γ- tocopherol of opertaion ending | 0.84 ug/ml | Standard Deviation 0.1 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | γ- tocopherol of 1days post surgery | 0.76 ug/ml | Standard Deviation 0.11 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | γ- tocopherol of 3days post surgery | 0.70 ug/ml | Standard Deviation 0.13 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | α- tocopherol of before induction | 12.67 ug/ml | Standard Deviation 1.96 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | α- tocopherol of clamping removal | 6.13 ug/ml | Standard Deviation 1.81 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | α- tocopherol of before induction | 12.22 ug/ml | Standard Deviation 1.83 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | α- tocopherol of clamping removal | 6.12 ug/ml | Standard Deviation 1.68 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | γ- tocopherol of clamping removal | 0.57 ug/ml | Standard Deviation 0.09 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | α- tocopherol of opertaion ending | 5.91 ug/ml | Standard Deviation 1.68 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | γ- tocopherol of 3days post surgery | 0.62 ug/ml | Standard Deviation 0.09 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | α- tocopherol of 1days post surgery | 8.23 ug/ml | Standard Deviation 1.51 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | γ- tocopherol of opertaion ending | 0.75 ug/ml | Standard Deviation 0.1 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | α- tocopherol of 3days post surgery | 9.31 ug/ml | Standard Deviation 1.56 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | γ- tocopherol of 7days post surgery | 0.60 ug/ml | Standard Deviation 0.11 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | α- tocopherol of 7days post surgery | 10.98 ug/ml | Standard Deviation 1.8 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | γ- tocopherol of 1days post surgery | 0.69 ug/ml | Standard Deviation 0.1 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress Confirmed by High Performance Liquid Chromatography | γ- tocopherol of before induction | 0.53 ug/ml | Standard Deviation 0.08 |
Primary
Evidences of Clinically Definite Oxidative Stress:Micronuclei
Evidences of clinically definite oxidative stress:micronuclei confirmed by Cytokinesis-block Micronucleus Test
Time frame: before induction,clamping removal ,operation ending,1,3,7days post surgery
| Arm | Measure | Group | Value (MEAN) | Dispersion |
|---|---|---|---|---|
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress:Micronuclei | micronuclei of 7days post surgery | 201.33 number of micronuclei/1000 BN cells | Standard Deviation 28.98 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress:Micronuclei | micronuclei of before induction | 185.83 number of micronuclei/1000 BN cells | Standard Deviation 21.69 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress:Micronuclei | micronuclei of clamping removal | 194.57 number of micronuclei/1000 BN cells | Standard Deviation 25.27 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress:Micronuclei | micronuclei of operation ending | 230.77 number of micronuclei/1000 BN cells | Standard Deviation 25 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress:Micronuclei | micronuclei of 1days post surgery | 278.73 number of micronuclei/1000 BN cells | Standard Deviation 30.86 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress:Micronuclei | micronuclei of 3days post surgery | 248.43 number of micronuclei/1000 BN cells | Standard Deviation 30.88 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress:Micronuclei | micronuclei of 1days post surgery | 402.27 number of micronuclei/1000 BN cells | Standard Deviation 27.6 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress:Micronuclei | micronuclei of 7days post surgery | 243.63 number of micronuclei/1000 BN cells | Standard Deviation 29.59 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress:Micronuclei | micronuclei of operation ending | 375.87 number of micronuclei/1000 BN cells | Standard Deviation 30.9 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress:Micronuclei | micronuclei of before induction | 186.40 number of micronuclei/1000 BN cells | Standard Deviation 19.87 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress:Micronuclei | micronuclei of 3days post surgery | 338.00 number of micronuclei/1000 BN cells | Standard Deviation 30.85 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress:Micronuclei | micronuclei of clamping removal | 195.73 number of micronuclei/1000 BN cells | Standard Deviation 24.21 |
Primary
Evidences of Clinically Definite Oxidative Stress: Nuclear Buds
Evidences of clinically definite oxidative stress:nuclear buds Confirmed by Cytokinesis-block Micronucleus Test
Time frame: before induction,clamping removal ,operation ending,1,3,7days post surgery
| Arm | Measure | Group | Value (MEAN) | Dispersion |
|---|---|---|---|---|
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress: Nuclear Buds | nuclear buds of before induction | 50.40 number of nuclear buds/1000 BN cells | Standard Deviation 8.84 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress: Nuclear Buds | nuclear buds of clamp ing removal | 54.33 number of nuclear buds/1000 BN cells | Standard Deviation 8.56 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress: Nuclear Buds | nuclear buds of operation ending | 61.10 number of nuclear buds/1000 BN cells | Standard Deviation 9.16 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress: Nuclear Buds | nuclear buds of 1days post surgery | 62.73 number of nuclear buds/1000 BN cells | Standard Deviation 10.38 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress: Nuclear Buds | nuclear buds of 3days post surgery | 58.00 number of nuclear buds/1000 BN cells | Standard Deviation 8.31 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress: Nuclear Buds | nuclear buds of 7days post surgery | 54.87 number of nuclear buds/1000 BN cells | Standard Deviation 9.56 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress: Nuclear Buds | nuclear buds of 3days post surgery | 61.70 number of nuclear buds/1000 BN cells | Standard Deviation 10.03 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress: Nuclear Buds | nuclear buds of before induction | 53.93 number of nuclear buds/1000 BN cells | Standard Deviation 9.11 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress: Nuclear Buds | nuclear buds of 1days post surgery | 65.07 number of nuclear buds/1000 BN cells | Standard Deviation 11.27 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress: Nuclear Buds | nuclear buds of clamp ing removal | 57.43 number of nuclear buds/1000 BN cells | Standard Deviation 8.44 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress: Nuclear Buds | nuclear buds of 7days post surgery | 55.67 number of nuclear buds/1000 BN cells | Standard Deviation 11.49 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress: Nuclear Buds | nuclear buds of operation ending | 62.87 number of nuclear buds/1000 BN cells | Standard Deviation 10.47 |
Primary
Evidences of Clinically Definite Oxidative Stress: Nucleoplasmic Bridges
Evidences of clinically definite oxidative stress: nucleoplasmic bridges confirmed by Cytokinesis-block Micronucleus Test
Time frame: before induction,clamping removal ,operation ending,1,3,7days post surgery
| Arm | Measure | Group | Value (MEAN) | Dispersion |
|---|---|---|---|---|
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress: Nucleoplasmic Bridges | nucleoplasmic bridges of before induction | 66.50 number of nucleoplasmic bridges/1000 BN | Standard Deviation 9.92 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress: Nucleoplasmic Bridges | nucleoplasmic bridges of clamp ing removal | 78.77 number of nucleoplasmic bridges/1000 BN | Standard Deviation 13.66 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress: Nucleoplasmic Bridges | nucleoplasmic bridges of operation ending | 88.40 number of nucleoplasmic bridges/1000 BN | Standard Deviation 14.08 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress: Nucleoplasmic Bridges | nucleoplasmic bridges of 1days post surgery | 96.83 number of nucleoplasmic bridges/1000 BN | Standard Deviation 14.74 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress: Nucleoplasmic Bridges | nucleoplasmic bridges of 3days post surgery | 86.87 number of nucleoplasmic bridges/1000 BN | Standard Deviation 11.29 |
| Propofol Postconditioning | Evidences of Clinically Definite Oxidative Stress: Nucleoplasmic Bridges | nucleoplasmic bridges of 7days post surgery | 79.20 number of nucleoplasmic bridges/1000 BN | Standard Deviation 11.17 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress: Nucleoplasmic Bridges | nucleoplasmic bridges of 3days post surgery | 96.37 number of nucleoplasmic bridges/1000 BN | Standard Deviation 12.7 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress: Nucleoplasmic Bridges | nucleoplasmic bridges of before induction | 68.73 number of nucleoplasmic bridges/1000 BN | Standard Deviation 8.22 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress: Nucleoplasmic Bridges | nucleoplasmic bridges of 1days post surgery | 111.93 number of nucleoplasmic bridges/1000 BN | Standard Deviation 11.77 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress: Nucleoplasmic Bridges | nucleoplasmic bridges of clamp ing removal | 80.80 number of nucleoplasmic bridges/1000 BN | Standard Deviation 12.34 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress: Nucleoplasmic Bridges | nucleoplasmic bridges of 7days post surgery | 87.83 number of nucleoplasmic bridges/1000 BN | Standard Deviation 12.85 |
| Sevoflurane | Evidences of Clinically Definite Oxidative Stress: Nucleoplasmic Bridges | nucleoplasmic bridges of operation ending | 96.23 number of nucleoplasmic bridges/1000 BN | Standard Deviation 12.7 |
Secondary
Mini Mental State Examination (MMSE)
A questionnaires is used to assess the cognitive function of patients in clinical,the total range was 0-30,and 27-30 were considered as normal value,\<27 were considered as recognitive dysfunction.
Time frame: before induction,1,3,7days post surgery
| Arm | Measure | Group | Value (MEAN) | Dispersion |
|---|---|---|---|---|
| Propofol Postconditioning | Mini Mental State Examination (MMSE) | 1days post surgery of MMSE | 23.40 units on a scale | Standard Deviation 1.87 |
| Propofol Postconditioning | Mini Mental State Examination (MMSE) | 3 days post surgery of MMSE | 24.43 units on a scale | Standard Deviation 2.18 |
| Propofol Postconditioning | Mini Mental State Examination (MMSE) | 7 days post surgery of MMSE | 27.00 units on a scale | Standard Deviation 2 |
| Propofol Postconditioning | Mini Mental State Examination (MMSE) | before induction of MMSE | 27.63 units on a scale | Standard Deviation 1.43 |
| Sevoflurane | Mini Mental State Examination (MMSE) | before induction of MMSE | 27.23 units on a scale | Standard Deviation 1.63 |
| Sevoflurane | Mini Mental State Examination (MMSE) | 1days post surgery of MMSE | 17.73 units on a scale | Standard Deviation 2.02 |
| Sevoflurane | Mini Mental State Examination (MMSE) | 7 days post surgery of MMSE | 24.30 units on a scale | Standard Deviation 1.97 |
| Sevoflurane | Mini Mental State Examination (MMSE) | 3 days post surgery of MMSE | 20.33 units on a scale | Standard Deviation 1.94 |
Secondary
Montreal Cognitive Assessment (MoCA)
A questionnaires is used to assess the cognitive function of patients in clinical,the total range was 0-30,and 27-30 were considered as normal value,\<27 were considered as recognitive dysfunction.
Time frame: before induction,1,3,7days post surgery
| Arm | Measure | Group | Value (MEAN) | Dispersion |
|---|---|---|---|---|
| Propofol Postconditioning | Montreal Cognitive Assessment (MoCA) | before induction of MoCA | 20.43 units on a scale | Standard Deviation 2.39 |
| Propofol Postconditioning | Montreal Cognitive Assessment (MoCA) | 1days post surgery | 21.53 units on a scale | Standard Deviation 2.58 |
| Propofol Postconditioning | Montreal Cognitive Assessment (MoCA) | 3days post surgery | 22.53 units on a scale | Standard Deviation 2.68 |
| Propofol Postconditioning | Montreal Cognitive Assessment (MoCA) | 7days post surgery | 26.20 units on a scale | Standard Deviation 2.27 |
| Sevoflurane | Montreal Cognitive Assessment (MoCA) | 7days post surgery | 23.10 units on a scale | Standard Deviation 2.73 |
| Sevoflurane | Montreal Cognitive Assessment (MoCA) | before induction of MoCA | 19.87 units on a scale | Standard Deviation 2.6 |
| Sevoflurane | Montreal Cognitive Assessment (MoCA) | 3days post surgery | 20.80 units on a scale | Standard Deviation 2.73 |
| Sevoflurane | Montreal Cognitive Assessment (MoCA) | 1days post surgery | 19.20 units on a scale | Standard Deviation 2.31 |