Inflammation
Conditions
Keywords
Air Pollution, Biodiesel exhaust, Bronchoscopy, Metabolomics
Brief summary
Air pollution is a global environmental and health concern, contributing to onset and deterioration of respiratory and cardiovascular diseases. As climate change and dependence on diminishing fossil fuel supplies have taken center stage in political and scientific debates, renewable carbon-neutral fuels like biodiesel receive increasing attention. The most common biodiesel within the European Union, rapeseed oil methyl ester (RME) is perceived to be a green fuel, as it is sustainable and of biological origin, and therefore is often predicted to be less harmful to human health. Whilst replacing petrodiesel with biodiesel may have advantageous ecological impacts, consequences to respiratory health remained largely unexplored. The purpose of the current study is to evaluate whether inhalation of 100% RME biodiesel exhaust would result in an acute airway inflammatory response in healthy human subjects, as shown previously following exposure to petrodiesel exhaust.
Interventions
OTHERBronchoscopy
The bronchoscopy procedure was performed six hours after completion of the exposure session, with sampling of endobronchial mucosal biopsies, bronchial wash and bronchoalveolar lavage performed under topical anaesthesia.
Sponsors
Umeå University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
20 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
Normal ECG Normal Lung function Normal Blood samples
Exclusion criteria
Smoking Pre-existing metabolomic, respiratory and cardiovascular disease Exposure to high levels of pollutants in occupation
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Cytokines in Bronchial wash (BW) | 6 hours | Bronchial wash (BW) collected by bronchoscopy will be analyzed for cytokines (%). |
| Cell counts in Bronchial Wash | 6 hours | Bronchial wash (BW) collected by bronchoscopy will be analyzed for inflammatory cell counts (cells/ml). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Oxylipin Metabolomic profiling | Baseline, 2 hours, 6 hours, 24 hours | Metabolomic testing done with mass spectrometry on blood samples, BW and BAL. |
| Inflammation in bronchoalveolar lavage (BAL) | 6 hour | BAL collected by bronchoscopy will be analyzed for inflammatory markers, such as cell counts (cells/ml). |
| Inflammation in endomucosal biopsies | 6 hours | Biopsies collected by bronchoscopy will be analyzed for inflammatory markers, such as cell counts (cells/mm2). |
Outcome results
None listed