Methodological Evaluation of Gingival Crevicular Fluid Volume and Elastase Level

Methodological Evaluation of Gingival Crevicular Fluid Volume and Elastase Levels: Sequential Sampling, Length of Sampling Time And Two Different Sampling Methods

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02676362

Enrollment

Registered

2016-02-08

Start date

2008-03-31

Completion date

2008-12-31

Last updated

2016-02-09

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Chronic Periodontitis

Brief summary

The mechanisms underlying the formation and composition of gingival crevicular fluid (GCF) and its flow into and from periodontal pockets are not understood very well. The aim of this study was to evaluate the length of sampling time and sequential sampling of GCF neutrophil elastase (NE) enzyme activity by using intracrevicular and orifice methods.

Detailed description

Twenty adults (mean age of 41.8 years. ranged 31-60 years. 18 males. 2 females) with chronic periodontitis were enrolled and all completed the 3-day study. Three-hundred-sixty samples of GCF were harvested from the interproximal sites of each of the two maxillary non-molar teeth according to the orifice method. Each site was sampled three times with 1-min intervals between repeat samples. By using intracrevicular method, the other 360 samples of GCF were collected on the same sites following 10-minutes interval. In first day, 2nd day and 3rd day the length of sampling time in seconds and order were 5. 10. 30; 10. 30. 5 and 30. 5. 10. respectively. GCF elastase activity was determined by hydrolysis of neutrophil specific substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide and was expressed as concentration (microU/microl) and total enzyme activity (microU).

Interventions

OTHERGingival crevicular fluid collection with filter paper

Gingival Crevicular Fluid Dynamics

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

DIAGNOSTIC

Masking

DOUBLE (Investigator, Outcomes Assessor)

Eligibility

Sex/Gender

ALL

Age

31 Years to 60 Years

Healthy volunteers

Yes

Inclusion criteria

* no history of systemic disease; * no history of antibiotics and/or anti-inflammatory drugs within the past 6 months; * no received any oral hygiene prophylaxis and scaling and root planing within the past 6 months; * at least 2 interproximal sites exhibiting higher than 5 mm probing depth, radiographic bone loss and gingival index of 2 (with preference of two maxillary non-molar teeth)

Exclusion criteria

* with metabolic diseases * pregnancy, lactation etc.

Design outcomes

Primary

Measure	Time frame
The length of sampling time of GCF Elastase Levels	3 days

Secondary

Measure	Time frame
The length of sampling time of GCF Volume	3 days
The length of sequential sampling of GCF Volume	3 days
The length of sequential sampling of GCF Elastase Levels	3 days

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026