Microswimup Procedure Efficacy
Conditions
Brief summary
The aim of this study is to evaluate the microbiological safety and the efficacy of a new not expensive, easy and fast technique (Direct Microswimup) for sperm preparation in In Vitro Fertilization (IVF) treatments without the use of centrifuge, able to simplify the procedure and to avoid mismatches by ensuring the same results of the standard techniques in terms of fertilization rate, blastulation rate, pregnancy/abortion rate, cryostorage rate, and a better euploid status of the embryos.
Interventions
None listed
Sponsors
Cervesi Hospital, Cattolica, Italy
Study design
Observational model
COHORT
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
20 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* at least 8 oocytes retrieved at pickup * oocyte insemination with ICSI technique * only elective single embryo transfer (eSET) at blastocyst stage * sperm concentration ≥ 1 X 106 /ml * absence of antibiotic treatment in the previous four weeks for male patients
Exclusion criteria
* recurrent miscarriages * more than three in vitro fertilization treatment * azoospermia
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| microbiological test | 3 months | A standard bacteriological culture \[based on inoculation of blood and chocolate agar plates and Brain-Heart Infusion broth, followed by incubation at 37°C for up to 72 hours\] was performed in order to investigate the presence and amount of colonizing bacteria delivered with the semen at ejaculation and after treatment |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| semen treatment efficacy | 6 months | number of obtain blastocysts (%) and number of pregnancy (%) |
Other
| Measure | Time frame | Description |
|---|---|---|
| euploid status | 6 months | molecular analysis of blastocyst's biopsy |
Countries
Italy
Outcome results
None listed