Oxidative Stress
Conditions
Brief summary
The purpose of this study is investigate to the effect(s) of chronic occupational exposure to N20 and volatile anesthetics on the extent of oxidative stress (DNA damage and the generation of the biochemical markers) in operating room staff, with different levels of exposure to halogenated hydrocarbons, in comparison to unexposed controls (Intensive Care Staff), and to determine the relationship between the degree of oxidative stress and psychological stress, as assessed by validated psychometric measures.
Detailed description
This is a single blinded randomized, controlled and prospective clinical trial comparing oxidative stress status in two groups. Subjects in two groups will be matched for gender, age, smoking habit and employment duration and will be randomized into one of the two groups of: 1. Pediatric OR Staff, and 2. control (e.g; ICU staff) Biomarkers of oxidative stress will be measured in two different time points in each subject, (Monday Morning and, Friday afternoon of that same week-\>40 hours in Operating room or Intensive Care Unit). The relationship between oxidative stress and psychological stress will also be assessed by validated psychometric measures.
Interventions
OTHERExposure to nitrous oxide or other volatile anesthetics
No intervention, but routine work-place exposure to volatile anesthetics
Sponsors
Nationwide Children's Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
Yes
Inclusion criteria
1. CHOR-Staff who work \> 40 hours per week in the operating rooms (Anesthesiologists, CRNA, Surgeons, Nurses) 2. NOR- Intensive Care Unit Staff who work \> 40 hours per week in Intensive care unit (Physicians, Nurses, APN'S)
Exclusion criteria
Staff with the following history will be excluded: 1. Therapeutic exposure to radiation 2. On chemotherapeutic agents 3. History of General Anesthesia during the past two years
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Oxidative Stress | During exposure to volatile anesthetics over the course of a week in the work-place | The extent of oxidative stress (DNA damage and the generation of the biochemical markers) in operating room staff with different levels of exposure to halogenated hydrocarbons, in comparison to unexposed controls. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Psychological Stress | During exposure to volatile anesthetics over the course of a week in the work-place | To determine the relationship between oxidative stress and psychological stress, as assessed by validated psychometric measures. |
Countries
United States
Outcome results
None listed