Aortic Dissection
Conditions
Keywords
Aortic dissection, Aldehyde dehydrogenase 2 polymorphism, Risk factor, aldehyde dehydrogenase 2
Brief summary
The purpose of this study is to evaluate the impact of ALDH2 Glu504Lys polymorphism on aortic dissection.
Interventions
None listed
Sponsors
Qilu Hospital of Shandong University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* Chinese Han people diagnosed with aortic dissection in Qilu Hospital
Exclusion criteria
* Aortic dissection based on trauma, connective tissue diseases and pregnancy * Patients with tumor * Patients with no written consensus
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Number of patients with different ALDH2 genotype | 1-30 days after samples collected | DNA was extracted from venous blood;genomic DNA underwent PCR amplification; finally, PCR products were purified and directly sequenced by Invitrogen Corp or Biosune Corp.Then ALDH2 genotype was distinguished depend on a G-to-A missense mutation on exon 12 of ALDH2 gene. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Concentration of inflammatory factors in blood and aortic specimen | 1-30 days after samples collected | Concentration of 4-HNE、MMP-2、MMP-9 detected by elisa kit、IHC or western blot in relevant specimen. |
| ALDH2 activity | 1-30 days after samples collected | Activity of ALDH2 was detected in blood and aortic specimen depend on its reducibility of NAD+ |
Countries
China
Outcome results
None listed