Oxidative Stress
Conditions
Keywords
Oxidative Stress, Oxygen, General Anesthesia
Brief summary
Clinical trial controlled randomized of parallels groups. The study is designed to look for differences in the oxidative stress level among healthy patients receiving 2 different oxygen concentrations: 30% and 60% during general anesthesia. Once the patient has been informed, the affirmative agreement has been obtained and inclusion and exclusion criteria verified the patients are randomized to 30% or 60% oxygen concentration. In the operating room while placing the intravenous line, a sample blood is obtained. General anesthesia is induced and maintained with sevoflurane. After Anesthesia induction the anesthesia machine is fixed in order to provide the oxygen concentration according to the randomization. One hour later a second venous sample is obtained. Samples are stored in cold conditions until their analysis in the laboratories of the biology faculty - Barcelona University.
Interventions
DRUG30% of oxygen concentration.
30% oxygen concentration during one hour of general anesthesia.
DRUG60% of oxygen concentration.
60% oxygen concentration during one hour of general anesthesia.
Sponsors
University of Barcelona
Fundació Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
25 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Elective surgery, under general anesthesia. * ASA 1 - 2 * Orl surgery, reconstructive surgery, neck surgery, abdominal wall surgery.
Exclusion criteria
* Smoking Habit * Pregnancy * No compensated disease. * ASA 3 or Higher.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in Malondialdehyde level | Baseline and after one hour of general anesthesia | A blood sample is obtained before general anesthesia and a second sample is obtained one hour after the oxygen concentration has been fixed in the anesthesia machine. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in proteic oxidation level | Baseline and after one hour of general anesthesia. | A blood sample is obtained before general anesthesia and a second sample is obtained one hour after the oxygen concentration has been fixed in the anesthesia machine. |
| Change in glutathion level | Baseline and after one hour of general anesthesia. | A blood sample is obtained before general anesthesia and a second sample is obtained one hour after the oxygen concentration has been fixed in the anesthesia machine. |
Countries
Spain
Contacts
Primary ContactSebastian Castrillon, M.D
Outcome results
None listed