Obesity, Insulin Resistance, Healthy
Conditions
Brief summary
The study will compare plasma and urine post-prandial metabolomics after fat and glucose oral load according to lifestyle factors.
Detailed description
The metabolic response in plasma and urine, pre- and post-load tests in people with specific dietary lifestyles will be tested. This project will focus on fat and glucose induced changes of targeted metabolic pathways that have been related to diabetes and obesity risk.
Interventions
Standardized oral glucose tolerance test (75 g oral glucose) and blood collection before and 2hrs after the glucose load
OTHERFat tolerance test
Oral fat tolerance will be provided to fasting participants and blood samples will be collected before and 4 hrs after the fat load.
Sponsors
Universität des Saarlandes
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
DIAGNOSTIC
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
40 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy volunteers * On a stable dietary habits
Exclusion criteria
* Pregnancy * Malabsorption disorders * Present or former cancer * Any oral medication that affect glucose or fat absorptions * Cholesterol lowering drugs (i.e. Statins) * other drugs: fibrates, MTX, or vitamin supplements * Diabetes (type 1 and type 2) * Alcoholism * Weight losing diet during the last 4 months * Liver diseases * Renal dysfunction * Gastric resection.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Plasma levels of branched chain amino acid | Changes between baseline and 2 hours |
| Plasma Trimethylamin N-oxide | Changes between baseline and 2 hours |
| Plasma glucose level | Changes between baseline and 2 hours |
| Plasma glucose | Changes between baseline and 2 hours |
| Plasma level of acetyl-CoA | Changes between baseline and 2 hours |
Secondary
| Measure | Time frame |
|---|---|
| Plasma malonyl-CoA | Changes between baseline and 2 hours |
| Plasma pyruvate | Changes between baseline and 2 hours |
| Plasma phosphatidylcholine | Changes between baseline and 2 hours |
| Plasma insulin | Changes between baseline and 2 hours |
| Plasma leptin | Changes between baseline and 2 hours |
| Plasma Lactate | Changes between baseline and 2 hours |
Countries
Germany
Outcome results
None listed