Acute Pancreatitis
Conditions
Keywords
acute pancreatitis, non-coding RNA, biological markers, receiver operating characteristic curve, post-ERCP pancreatitis
Brief summary
It is important to identify patients with acute pancreatitis who are at risk for developing persistent organ failure early in the course of disease. The investigators evaluated whether peripheral blood non-coding RNAs, including microRNAs and long noncoding RNA (lncRNA), could serve as a good marker for detection of acute pancreatitis with persistent organ failure at early phase.
Detailed description
Acute pancreatitis is a sudden inflammation of the pancreas. It can have severe complications and high mortality despite treatment. While mild cases are often successfully treated with conservative measures, such as fasting and aggressive intravenous fluid rehydration, severe cases may require admission to the intensive care unit or even surgery to deal with complications of the disease process. The diagnosis of severe acute pancreatitis at an early phase remain a major challenge for clinicians. Therefore, many biologic markers have been studied in an effort to improve the diagnostic rate and determine the severity of acute pancreatitis but with disappointing results. Non-coding RNAs, including microRNAs and long noncoding RNA (lncRNA), have recently been validated to stably exist in peripheral blood. Several publications showed that it may serve as potential markers for various diseases, including cancer and inflammation. Our current study evaluated whether and which kind of non-coding RNAs could serve as good markers for severe acute pancreatitis.
Interventions
None listed
Sponsors
Changhai Hospital
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patients who was diagnosed acute pancreatitis * Male or female * 18 Years and older * written informed consent
Exclusion criteria
* Symptoms of acute pancreatitis present for more than 72 hours * Age under 18 years * Pregnancy * patients unable to consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| The level of non-coding RNAs in peripheral blood | At admission | RNA quantification after extraction using RT-PCR |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| serum urea nitrogen | At admission | serum urea nitrogen quantification |
| serum creatinine | At admission | serum creatinine quantification |
| APACHE II score at admission | At admission | APACHE II score determined by Temperature, MAP, heart rate, respiratory rate, Pao2, arterial pH, HCO3, sodium, potassium, creatinine, hematocrit, WBC, Glasgow Coma Score, age, chronic health points |
| BISAP score at admission | At admission | BISAP socre determined by BUN (\>25 mg/dL), impaired mental status (Glasgow Coma Score \<15), SIRS (≥2), age (\>60 y), pleural effusion |
Countries
China
Outcome results
None listed