Healthy
Conditions
Keywords
fructose, metabolism, acute
Brief summary
The purpose of this study is to investigate the metabolic and endocrine responses during 3 hour after taking 75g fructose or glucose in young Chinese.
Detailed description
The study was a double-blinded, randomized, crossover trial. Twenty healthy volunteers were randomized to two groups: group A (300ml of 22.5% glucose) and group B (300ml of 22.5% fructose).Volunteer was randomized into either group A or B, after an 8-h overnight fasting, took 300ml of solution at 0830 within 5 minutes. After one-week washout period, volunteer was crossed over for either group B or A. each intervention, venous blood was collected at 0h, 0.5h, 1h, 2h, and 3h and blood pressure was tested at 0h, 1h, 2h, and 3h.
Interventions
DIETARY_SUPPLEMENTfructose
75g fructose is dissolved in 300 ml water.After overnight fasting, 300ml of solution at 0830 within 5 minutes is taken by volunteers
DIETARY_SUPPLEMENTglucose
75g glucose is dissolved in 300 ml water.After overnight fasting, 300ml of solution at 0830 within 5 minutes is taken by volunteers
Sponsors
Zhejiang University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
TRIPLE (Subject, Caregiver, Investigator)
Eligibility
Sex/Gender
ALL
Age
19 Years to 39 Years
Healthy volunteers
Yes
Inclusion criteria
* Inclusion criteria were aged 19-39 years, with BMI between 18-24 kg/m 2.
Exclusion criteria
* Subjects pregnant or having a history of hepatic or renal disease, fructose intolerance, gout, hypoglycemia, or diabetes mellitus were excluded. * Subjects taking any drugs, botanicals, or nutraceutical known to influence blood pressure also were rejected either.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| serum uric acid | 3 hours | Serum uric acid concentration was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by DiaSys Diagnostic Systems (Shanghai) Co., Ltd |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| blood pressure | 3 hours | Blood pressure was measured at Zhejiang University Hospital by the research workers who were blind to the groups within 3 hours blood collection. |
Other
| Measure | Time frame | Description |
|---|---|---|
| aldose reductase | 3 hours | Enzymatic activity of aldose reductase in serum was measured using ELISA kit (Shanghai Changjin Biology Co., Lte., Shanghai, China ) by the research workers who were blind to the groups. |
| phosphofructokinase | 3 hours | Enzymatic activity of phosphofructokinase in serum was measured using ELISA kit (Shanghai Changjin Biology Co., Lte., Shanghai, China ) by the research workers who were blind to the groups . |
| insulin | 3 hours | Serum insulin concentration was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd |
| insulin-c | 3 hours | Serum insulin-c concentration was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd |
| serum glucose | 3 hours | Serum glucose level was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd |
| total cholesterol | 3 hours | Total cholesterol concentration was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd |
| high-density cholesterol | 3 hours | High-density cholesterol was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd. |
| low-density cholesterol | 3 hours | Low-density cholesterol concentration was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd. |
| lactate dehydrogenase | 3 hours | Enzymatic activity of lactate dehydrogenase was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd. |
| triglyceride | 3 hours | Triglyceride concentration in serum was analyzed on HITACHI 7020 chemistry analyzer using enzyme-based colorimetric test or colorimetric test supplied by Diasys Diagnostic Systems (Shanghai) Co., Ltd |
| nitric oxide | 3 hours | Serum nitric oxide concentration was measured using NO assay kit (Nanjing Jiancheng Technology Co., Lte., Nanjing, China) by the research workers who were blind to the groups. |
Countries
China
Outcome results
None listed