Primary Culture of Residual Specimens Obtained From Aspiration of Hepatic Tumor to Predict the Prognosis of the Patients

Kaohsiung Medical University Chung-Ho Memorial Hospital

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02587793

Enrollment

208

Registered

2015-10-27

Start date

2014-10-31

Completion date

2018-07-31

Last updated

2019-02-27

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Hepatocellular Carcinoma

Keywords

Hepatocellular Carcinoma, primary culture, fine-needle aspiration, cancer-associated fibroblast

Brief summary

The aim of this project is to investigate whether the proliferative speeds of cultured cells using our method can be integrated into Barcelona Clinic Liver Cancer (BCLC) staging classification for the possibility of personalized treatment and prediction the outcomes in hepatocellular carcinoma (HCC) patients.

Detailed description

A total of at least 150 patients will be included and followed for at least one year. The proliferative speeds of cultured cells will be classified into (a) rapid proliferation of HCC cells with or without concomitant rapid proliferation of cancer-associated fibroblasts (CAFs), (b) rapid proliferation of CAFs alone, and (c) slow proliferation three groups. The definition for the rapid proliferation of cultured cells fits at least one of the following two items: (1) growth area of cultured cells at the 15th-28th day \> two times of the growth area measured at the 14th day, or (2) growth area of cultured cells at the 15th-28th day \> 70% growth area of the 25 cm flask. The GraphPad Prism software version 4.03 (GraphPad Software, Inc., La Jolla, CA, USA) is applied for statistical analysis. Influence of the proliferative speeds of cultured cells on survival and cancer progression will be calculated by the logrank test. The statistical significance is defined as P \< 0.05.

Interventions

OTHERnot interventional study

Study design

Observational model

CASE_ONLY

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Healthy volunteers

Inclusion criteria

1. Patients are proven to have HCC based on fine-needle aspiration of the tumor. 2. Patient have residual specimens obtained from fine-needle aspiration of the tumor. 3. Patients agree to participate this study after fully explanation.

Exclusion criteria

1. Patients are not proven to have HCC based on fine-needle aspiration of the tumor. 2. Patient do not have residual specimens obtained from fine-needle aspiration of the tumor. 3. Patients do not agree to participate this study after fully explanation.

Design outcomes

Primary

Measure	Time frame	Description
survival	one year	Correlation between the proliferative speeds of cultured cells and one year survival of the patients after aspiration of the tumor will be investigated.

Secondary

Measure	Time frame	Description
cancer progression	one year	Correlation between the proliferative speeds of cultured cells and HCC progression one year after aspiration of the tumor will be investigated.

Countries

Taiwan

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026