Proteomic Analysis of Mononuclear Cells After a High-Fat, High-Carbohydrate Meal With or Without Orange Juice

Proteomic Analysis of the Peripheral-Blood Mononuclear Cells Response After a High-Fat, High-Carbohydrate Meal With or Without Orange Juice in Healthy Subjects

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02587507

Enrollment

Registered

2015-10-27

Start date

2015-06-30

Completion date

2015-10-31

Last updated

2016-07-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Inflammation

Brief summary

A high-fat, high-carbohydrate meal (HFHC) induces an increase in inflammation in peripheral-blood mononuclear cells in healthy subjects. A few studies have shown that orange juice, when consumed together with the high-fat meal, is able to revert the increase in inflammatory markers. The present study will assess the effect of a single HFHC meal taken with water, orange juice or an isocaloric glucose drink on protein expression in peripheral blood mononuclear cells of healthy individuals. Twelve healthy, normal weight individuals consumed a HFHC meal with water, water with glucose or orange juice in a cross-over design with at least one week interval between the meals. Blood samples were collected before, 1, 3 and 5h after the meals.

Detailed description

BACKGROUND: A high-fat, high-carbohydrate meal (HFHC) induces an increase in inflammation in peripheral-blood mononuclear cells in healthy subjects. A few studies have shown that orange juice, when consumed together with the high-fat meal, is able to revert the increase in inflammatory markers. OBJECTIVES: To study the effect of a single HFHC meal taken with water, orange juice (500ml) or an isocaloric glucose drink on protein expression in peripheral blood mononuclear cells of healthy individuals. DESIGN AND METHODOLOGY: Twelve healthy, normal weight individuals consumed a HFHC meal with water, water with glucose or orange juice in a cross-over design with at least one week interval between the meals. Blood samples were collected before, 1, 3 and 5h after the meals. Blood glucose, insulin and lipid profile were measured. Also, proteome analysis using TMT labeling was performed on peripheral-blood mononuclear cells collected before and at times 3 and 5h after the meal.

Interventions

OTHEROrange juice

Individuals will consume the HFHC meal with 500mL of orange juice.

OTHERWater

Individuals will consume the HFHC meal with 500mL of water.

OTHERWater with glucose

Individuals will consume the HFHC meal with 500mL of water with glucose.

Study design

Allocation

RANDOMIZED

Intervention model

CROSSOVER

Primary purpose

BASIC_SCIENCE

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

20 Years to 35 Years

Healthy volunteers

Yes

Inclusion criteria

* healthy * normal weight * non-vegetarian

Exclusion criteria

* use of medication * alcohol consumption, * intense physical exercise * pregnant or lactating * overweight * obese * drug abuse * any disease such as diabetes, inflammatory conditions.

Design outcomes

Primary

Measure	Time frame
Change in protein expression at mononuclear cells of healthy individuals before and after the high fat meal using TMT labeling and mass spectrometry	before the meal, 1h, 3h and 5h after the meal

Secondary

Measure	Time frame	Description
Blood glucose	before the meal	—
Insulin	before the meal	—
Lipid profile	before the meal	total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026