Tobacco Dependence, Vascular Complications, Vascular Stiffness
Conditions
Keywords
microvesicles, microparticles, Plethysmography, Snus, Arterial Stiffness, EPC
Brief summary
The current study proposes to investigate the effects of chronic snus use on the blood vessels. Several cardiovascular endpoints are measured using various methods in healthy daily snus users as well as in healthy matched controls.
Detailed description
The Swedish moist oral snuff known as snus has previously been found primarily in Scandinavia. In Sweden approximately 1 in 5 men are habitual users of snus. It is estimated that 3-4% of the female population are regular users. However, the last few years has seen a shift in the global tobacco industry towards finding novel ways of entering the smokeless tobacco (ST) markets. In the United States both Camel and Marlboro brands, among others, have launched an array of ST products similar to Swedish snus. Since its launch in 2007, the US snus market has continued to grow at an exponential rate and now sells approximately 50 million cans a year. Smoking has been studied extensively and been undoubtedly linked to a range of detrimental health effects, including cardiovascular disease. As snus has until recently been available in a limited Scandinavian market, few experimental and epidemiological studies have been performed. A recent meta-analysis found snus to be associated with heart failure and higher risk of fatal myocardial infarctions and stroke. Following myocardial infarction, discontinuation of snus use was associated with a 50% decreased risk of mortality. With new emerging markets worldwide publicizing the product as a discrete and healthier alternative to cigarettes, it has become all the more imperative to study the health effects of this smokeless tobacco. Using well validated methods the investigators intend to study the possible effects of chronic snus use on vascular endothelial function, thrombosis, circulating microparticles in blood and arterial stiffness.
Interventions
None listed
Sponsors
Umeå University
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
MALE
Age
30 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Normal clinical examination * Normal ECG * Normal routine blood test including serum lipids and HbA1C
Exclusion criteria
* Any form of cardiovascular disease * Any form of systemic or chronic disorder like rheumatologic or metabolic diseases. * Active allergy within 4 weeks of the study * Symptoms of infection or inflammation within 4 weeks of the study
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Venous occlusion plethysmography | 1 day | Venous occlusion plethysmography is a semi-invasive method of estimating vascular function in which brachial forearm blood flow is measured. In combination with intra-arterial infusion of substances with vasodilatory effects (for example bradykinin, sodium nitroprusside and acetylcholine) this method is considered the golden standard for assessing endothelial function. Measurements are given in ml/100 ml tissue/min. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Circulating microvesicles | 1 day | Blood sample, expressed in 10\^6MVs/L |
| Endothelial progenitor cells | 1 day | Blood sample, measured as CD34+KDR+(KDR:vascular endothelial growth factor receptor 2) double positive cells, and results are presented as the number of EPC events. |
| Arterial Stiffness | 1 day | PWA, PWV. Expressed as Pulse wave velocity (m/s) och augmentation index adjusted for pulse 75 (%). |
Countries
Sweden
Outcome results
None listed