Role of Colonic Short Chain Fatty Acids in Obesity

Effect of Inulin and Resistant Starch on Postprandial Short Chain Fatty Acid, Glucose, Insulin and Gut Hormone Responses in Lean and Overweight Subjects.

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02562014

Enrollment

Registered

2015-09-29

Start date

2012-02-29

Completion date

2013-01-31

Last updated

2015-09-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Obesity

Brief summary

The excess production of colonic short-chain-fatty-acids (SCFA) has been implicated in the promotion of obesity, but colonic fermentation of dietary-fiber to SCFA may also play a role in preventing diabetes. The investigators aimed to compare the effects of two fermentable fibers (inulin and resistant-starch) on postprandial SCFA, glucose, insulin, free-fatty acids (FFA) and gut hormone responses and to compare the responses in healthy overweight and obese (OWO) vs lean (LN) participants. Methods: Using a randomized, single blind, crossover design, 13 OWO and 12 LN overnight fasted participants were studied on 3 separate occasions. On each day they consumed a 300 mL drink containing 75g glucose (Control) or 75g glucose plus 24g inulin (IN), or 28.2g resistant-starch (RS). A standard lunch was served 4 h after the test drink.

Interventions

OTHERGlucose

75g glucose

OTHERInulin

75g glucose plus 24g inulin

OTHERResistant Starch

75g glucose plus 28.2g resistant starch

Study design

Allocation

RANDOMIZED

Intervention model

CROSSOVER

Primary purpose

BASIC_SCIENCE

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

18 Years to 65 Years

Healthy volunteers

Yes

Inclusion criteria

* non-pregnant * body mass index \>=20 and \<=35

Exclusion criteria

* history of diabetes mellitus * history of cardiovascular disease * history of bowel disease * history of kidney disease * history of liver disease * use of antibiotics within 3 months of enrolment * unusual dietary habits * fasting glucose \>6.9mmol/L * hematocrit below normal range for age and sex

Design outcomes

Primary

Measure	Time frame
Incremental area under the serum acetate response curve	For 6 hours after consuming the test meal

Secondary

Measure	Time frame
Incremental area under the breath methane response curve	For 6 hours after consuming the test meal
Incremental area under the serum insulin response curve	For 4 hours after consuming the test meal
Incremental area under the serum c-peptide response curve	For 4 hours after consuming the test meal
Total area under the serum free-fatty acid response curve	For 4 hours after consuming the test meal
Incremental area under the serum propionate response curve	For 6 hours after consuming the test meal
Incremental area under the serum butyrate response curve	For 6 hours after consuming the test meal
Incremental area under the breath hydrogen response curve	For 6 hours after consuming the test meal
Incremental area under the serum glucose response curve	For 4 hours after consuming the test meal

Other

Measure	Time frame
Incremental area under the serum ghrelin response curve	For 4 hours after consuming the test meal
Incremental area under the serum peptide-YY response curve	For 4 hours after consuming the test meal
Incremental area under the serum glucagon-like peptide-1 response curve	For 4 hours after consuming the test meal

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026