Rosacea
Conditions
Keywords
demodex, oxidative stress, reflectance confocal microscopy
Brief summary
Aim of study is to determine reactive oxygen products and antioxidant capacity with rosacea patients; to measure metalloproteinase level and inflammatory markers of immune response and investigate their corelation with demodex infestation and also investigate the role of photoaging and sebum secretion in rosacea.
Detailed description
Rosacea is a common and chronic inflammatory disease characterized by facial erythema, papule, pustule and telangiectasia. Ultraviolet, Demodex mite, oxidative stress and antioxidant system disorders are emphasized in increased inflammation seen in rosacea. The aim of our study was to evaluate the status of oxidative stress, matrix metalloproteinase (MMP) and inflammatory markers in systemic circulation, their relationship with demodex density and investigate the role of photoaging and sebum secretion in rosacea. Forty patients with rosacea and age, gender and skin phenotype matched 40 healthy volunteers were enrolled in the study. Reflectance confocal microscopy (RCM) was used to calculate facial demodex density. The number of demodex, follicle, the number of mite per follicle, the number of infested follicle and the number of mite per infested follicle were calculated with RCM. Photoaging severity and facial sebum levels were also assessed. Total oxidant capacity (TOC), total antioxidant capacity (TAC), oxidative stress index (OSI), paraoxonase-1 (PON-1), aryl esterase (ARES), myeloperoxidase (MPO), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-9 (MMP-9) levels in venous blood were measured.
Interventions
DEVICEVideo dermoscopy
Dermoscopic photoaging scale were assessed by using video dermoscopy.
OTHERObtaining a blood sample
Total oxidant capacity (TOC), total antioxidant capacity (TAC), oxidative stress index (OSI), paraoxonase-1 (PON-1), aryl esterase (ARES), myeloperoxidase (MPO), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-9 (MMP-9) levels in venous blood were measured.
1mm2-sized 10 images were taken from right cheek and forehead; the number of demodex, follicle, the number of mite per follicle, the number of infested follicle and the number of mite per infested follicle were calculated with RCM. And photoaging severity were also assessed by using RCM.
DEVICESebumeter
Sebum rate at forehead and right cheek were evaluated with sebumeter
Sponsors
Bezmialem Vakif University
Istanbul Training and Research Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* Between the ages of 18-70 * No systemic illness * No other inflammatory dermatoses except for rosacea * No smoking * Not using any topical, systemic treatment and sunblock at least a month for rosacea or other condition
Exclusion criteria
* Systemic illness * Other inflammatory dermatoses except for rosacea * Smoking * Using any topical, systemic treatment and sunblock for rosacea or other condition * Type 4 rosacea patient (only ocular involvement)
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Severity of rosacea | Rosacea patients who refer to criteria will be evaluated throughout 4 months. |
Secondary
| Measure | Time frame |
|---|---|
| The number of demodex of right cheek and forehead | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| The number of follicle of right cheek and forehead | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| The number of mite per follicle of right cheek and forehead | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| The number of infested follicle of right cheek and forehead | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| The number of mite per infested follicle of right cheek and forehead | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Total oxidant capacity (TOC) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Total antioxidant capacity (TAC) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Oxidative stress index (OSI) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Matrix metalloproteinase-1 (MMP-1) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Matrix metalloproteinase-1 (MMP-9) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Paraoxonase-1 (PON-1) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Aryl esterase (ARES) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Myeloperoxidase (MPO) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Tumor necrosis factor-alpha (TNF-α) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Interleukin-1beta (IL-1β) | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
Other
| Measure | Time frame |
|---|---|
| Sebum levels of right cheek and forehead | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
| Photoaging severity of face by using RCM and dermoscopy | Rosacea patients and healthy volunteers who refer to criteria will be evaluated throughout 4 months. |
Countries
Turkey (Türkiye)
Contacts
Primary ContactTugba Falay
Backup ContactVefa Aslı Erdemir
Outcome results
None listed