Microcirculation, Blood Circulation
Conditions
Keywords
microcirculation, confocal microscopy, hypocapnia
Brief summary
The microcirculatory alterations is common in circulatory failure, especially during sepsis. The severity of these changes and their sustainability are responsible of multi organ failure and ultimately death. The optimization of microcirculatory flow could be a central objective of the management of patients hospitalized in intensive care. Microcirculation includes all blood vessels of a diameter smaller than 100 micrometer. It represents the largest heat exchange surface of the body and is involved in tissue oxygenation. Microcirculatory flow is conditioned by the macrocirculation (heart rate and blood pressure) and the state of the microcirculation (thrombosis, vasoconstriction ...). The role of the CO2 in regulating microcirculatory flow is little studied. A recent work of our team and the oldest work in the literature lead to believe that CO2 has a specific role in modulating microcirculatory flow. No study to date precisely studied the impact of changes in the microcirculatory flow carbonemia . The hypocapnia test is carried out in a standardized manner by inhalation of a mixture enriched in CO2 7% allows a significant increase in carbonemia. Hypocapnia will in turn obtained by a calibrated voluntary hyperventilation test. Direct visualization of microcirculation by confocal microscopy is now considered the gold standard for exploring the microcirculation.
Interventions
DEVICEconfocal microscopy
microvessel diameter measured by confocal microscopy
Sponsors
Centre Hospitalier Universitaire de Saint Etienne
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* patients from achieving hypercapnia test the CHU of Saint-Etienne * non smoking * non diabetic * affiliated with or entitled to a social security system * Written consent
Exclusion criteria
* subjects not performing the test hypercapnia in full * patients with dermatological pathology at the study area * patient with Raynaud's syndrome * Patients with known bleeding disorders * refusal to consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| microcirculatory flow (cells / min) measured by confocal microscopy | Day1 | microcirculatory flow (cells / min) measured by confocal microscopy in hypocapnic patients |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| microvessel diameter (micrometer) measured by confocal microscopy | Day1 | microvessel diameter (micrometer) measured by confocal microscopy in hypocapnic patients |
Countries
France
Outcome results
None listed