Lipids
Conditions
Keywords
HDL biochemical composition and function
Brief summary
In this study the investigators aimed at measuring the effect of a two-week high-fat/high-cholesterol (HFHC) normocaloric diet on HDL particles biochemical composition and HDL function compared to a two-week low-fat/low-cholesterol (LFLC) normocaloric diet.
Detailed description
HDL particles can change their composition and function in response to a variety of pathological conditions and environmental stimuli. It has been previously shown that a western diet is able to drastically modify biochemical composition, anti-inflammatory and anti-oxidant properties of HDL in animals. In humans little is known on the role of diet on HDL function and composition.
Interventions
DIETARY_SUPPLEMENTLow fat/cholesterol diet
The low fat/cholesterol diet had 10% calories from fat and 150-200 mg of cholesterol per day.
DIETARY_SUPPLEMENTHigh fat/cholesterol diet
The high fat/cholesterol diet had 40% calories from fat and 250-300 mg of cholesterol per day
Sponsors
Azienda Ospedaliero, Universitaria Pisana
Study design
Allocation
RANDOMIZED
Intervention model
SINGLE_GROUP
Primary purpose
PREVENTION
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Subjects ≥ 18 and ≤40 years of age * Lean or overweight (BMI: 18 to 29,9 kg/m2) * Normal liver and kidney function * Normal thyroid function * Stable weight in the last 3 months * Read and understood the informed consent form and signed it voluntarily
Exclusion criteria
* Liver, heart, kidney, lung, infectious, neurological, psychiatric, endocrine, immunological or neoplastic diseases * Chronic use of drugs or vitamin supplements * Smoking * Pregnancy or lactation
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Dichlorofluorescein fluorescence slope | 14 days |
Secondary
| Measure | Time frame |
|---|---|
| hydroxyeicosatetraenoic acid | 14 days |
| hydroxyoctadecadienoic acid | 14 days |
Countries
Italy
Outcome results
None listed