Healthy
Conditions
Keywords
Berry, Inflammation, Cognition, Polyphenols, Appetite, Glucose metabolism
Brief summary
The main objective of this study is to investigate the effect of a 4-weeks intake of berry peel powder on fasting and postprandial glucose metabolism and inflammatory markers. The berry peel product will be compared with a placebo in a single-blind, cross-over design. Furthermore, inflammation-related peripheral blood mononuclear cells (PBMCs) genes expression, appetite and cognitive performance will be included as a pilot study. Investigators hypothesize that long-term consumption of polyphenols and fibres of berry peel will exhibit both direct and indirect actions in healthy overweight subjects by improvement of glucose-associated markers as well as ameliorating inflammation. Moreover, above metabolic markers will correlated with improvements in inflammation-related PBMCs genes expression, subjective appetite and cognitive performance after long-term consumption of berry peel as a source of polyphenols.
Interventions
DIETARY_SUPPLEMENTBerry peel drink
DIETARY_SUPPLEMENTControl drink
Sponsors
Conselho Nacional de Desenvolvimento Científico e Tecnológico
University of Campinas, Brazil
Lund University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy males and females * Signed informed consent * BMI 25-30 kg/m2 * Must be able to accept plant-based foods/drinks
Exclusion criteria
* Below 18 years or above 40 years * Smoking or using snuff * vegetarian or vegan * Stressed by venous blood sampling or previous experience of being difficult canulation
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Postprandial blood glucose response after acute intervention | -30, 0, 15, 30, 45, 60, 120, and 180 min after challenge meal. | The capillary blood will be collected for glucose analysis using a glucometer. |
| Incremental area under curve (iAUC) blood glucose response after long-term intervention | 4 weeks | The capillary blood will be collected for glucose analysis using a glucometer. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Postprandial subjective appetite rating after acute intervention | -30, 0, 15, 30, 45, 60, 120, and 180 min. | Appetite will be assessed using standard subjective 100mm visual analogue scaled (VAS). |
| Incremental area under curve (iAUC) subjective appetite rating after long-term intervention | 4 weeks | Appetite will be assessed using standard subjective 100mm visual analogue scaled (VAS). |
| Postprandial C-reactive protein (CRP) after acute intervention | -30, 60, 120, and 180 min. | The venous blood will be collected for CRP analysis using ELISA commercial kits |
| Incremental area under curve (iAUC) of C-reactive protein (CRP) after long-term intervention | 4 weeks | The venous blood will be collected for inflammatory analysis using ELISA commercial kits |
| Postprandial adiponectin after acute intervention | -30, 0, 60, 120, and 180 min. | The venous blood will be collected for adiponectin analysis using ELISA commercial kits |
| Postprandial serum insulin response after acute intervention | -30, 0, 15, 30, 45, 60, 120, and 180 min. | The capillary blood will be collected for insulin analysis using commercial ELISA kits |
| Peripheral blood mononuclear cells (PBMCs) inflammatory genes expression after acute intervention | 180 min after challenge meal. | The venous blood samples will be taken for isolation of peripheral blood mononuclear cells (PBMCs). Extracted total RNA will then be used for running quantitative-PCR. |
| Peripheral blood mononuclear cells (PBMCs) inflammatory genes expression after long-term intervention | -30 min. (Day-28 control); 180 min. (Day-28 control); -30 min. (Day-28 berry); 180 min. (Day-28 berry) | The venous blood samples will be taken for isolation of peripheral blood mononuclear cells (PBMCs). Extracted total RNA will then be used for running quantitative-PCR. |
| Postprandial selective attention after long-term intervention | 45, 90, and 180 min after challenge meal. | The selective attention will be assessed using a standardized software (Röd and Grön software) that measures wrong/right decisions and time of reaction when the subjects is faced with a challenging stimulus. |
| Postprandial interleukin-6 (IL-6) after acute intervention | -30, 60, 120, and 180 min. | The venous blood will be collected for IL-6 analysis using ELISA commercial kits |
| Incremental area under curve (iAUC) of interleukin-6 (IL-6) after long-term intervention | 4 weeks | The venous blood will be collected for IL-6 analysis using ELISA commercial kits |
| Postprandial adiponectin after long-term intervention with berry peel | 4 weeks | The venous blood will be collected for adiponectin analysis using ELISA commercial kits |
| Incremental area under curve (iAUC) serum insulin response after long-term intervention | 4 weeks | The capillary blood will be collected for insulin analysis using commercial ELISA kits |
Countries
Sweden
Outcome results
None listed