Healthy
Conditions
Keywords
endothelial dysfunction, microvesicles, endothelial progenitor cells, electronic cigarettes
Brief summary
We plan to investigate the acute effects of inhaling e-cigarette vapor on cell function measured by microvesicles and endothelial progenitor cells. Micro vesicles are released upon either activation or apoptosis from different cell types such as platelets, leucocytes and endothelial cell. Endothelial progenitor cells are a type of stem cells that circulate in the blood with the ability to differentiate to endothelial cells. Endothelial progenitor cells are inversely correlated to cardiovascular risk factors. As a secondary endpoint we plan to investigate exhaled nitric oxide - a common inflammation marker used in asthmatic patients - after inhalation of electronic cigarette vapor.
Interventions
DEVICEElectronic cigarette
Inhaling e-cigarette vapor
Sponsors
Karolinska Institutet
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* healthy subjects * sporadic cigarette smoking (\<9 cigarettes per month)
Exclusion criteria
* Any form of cardiovascular disease * Any form of pulmonary disease like asthma or COPD * Any form of systemic or chronic disorder like rheumatologic or metabolic diseases. * Active allergy within 4 weeks of the study * Symptoms of infection or inflammation within 4 weeks of the study * Pregnancy
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Change in number of endothelial progenitor cells | 24 hours |
| Change in number of micro vesicles | 24 hours |
Secondary
| Measure | Time frame |
|---|---|
| Change in exhaled nitric oxide | 24 hours |
Countries
Sweden
Outcome results
None listed