Skeletal Muscle Mass Regulation
Conditions
Keywords
Muscle mass, strength, NSAID, Ibuprofen, resistance exercise
Brief summary
In this study, healthy human subjects (18-35 yrs) are be randomised to consume 1200 mg ibuprofen or 75 mg acetylsalicylic acid daily during a training period of 8 weeks. During these weeks, subjects perform 2-3 supervised strength training sessions (knee extensions). Before and after training, muscle volume is measured by MRI, and muscle strength is assessed by isokinetic and isoinertial ergometers. Muscle biopsies are obtained from m. vastus lateralis to study gene and protein expression of markers regulating muscle protein turnover and prostaglandin synthesis.
Interventions
DRUGIbuprofen
DRUGAcetylsalicylic acid
Sponsors
Karolinska Institutet
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Considered healthy * Moderately trained * 18-35 yrs
Exclusion criteria
* Cardiovascular disease * Impaired kidney or liver function * Lower limb injury * Pregnancy * Ulcer or gastrointestinal upsets * Previous reactions to use of NSAID
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in Quadriceps muscle volume | Baseline and after 8 weeks training | Measured through MRI |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in muscle gene expression | Baseline, 4 weeks and after 8 weeks training | Through quantitative PCR and microarray |
| Change in muscle protein expression | Baseline, 4 weeks and after 8 weeks training | Through Western Blots |
| Change in Quadriceps muscle strength | Baseline and after 8 weeks training | Measured in strength dynamometers |
Countries
Sweden
Outcome results
None listed