Renal Tubular Transport
Conditions
Brief summary
Ghrelin (growth hormone release inducer) is produced in the stomach. The active form of Ghrelin is Acyl-Ghrelin. Acyl-Ghrelin stimulates the Ghrelin receptors. Ghrelin receptors are detected in the distal tubules in mice and animal studies have shown that Ghrelin increases the absorption of sodium in the renal tubules. Ghrelin infusion directly into the renal artery of rats increased sodium reabsorption in the distal nephron, presumably via the epithelial sodium channels (ENaC). The purpose of the study is to measure the acyl-ghrelin induced effects on GFR, tubular transport of sodium and water in different nephron segments and central and peripheral blood pressure in a randomized, cross-over, single-blind, placebo-controlled dose-response study.
Interventions
DRUGAcyl-Ghrelin
Sponsors
University of Aarhus
Erling Bjerregaard Pedersen
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Age 18-40 years, * BMI 18,5-30kg/m2, * women must use contraception.
Exclusion criteria
* Tobacco smoking, * substance abuse, * consumption of more than 7 units of alcohol/week for women and more than 14 units/week for men, * medical treatment except for contraception, * pregnancy or nursing, * allergy to acyl-ghrelin, * significant clinical signs of heart-, lung-, liver-, kidney-, endocrine-, brain- or neoplastic disorders, * clinically significant abnormal findings in screening blood samples, * urine sample or ECG, * office blood pressure over 140/90 mmHg, * donation of blood within 1 month of the first day of investigation.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Fractional sodium excreation (FENa) | 5-6 Hours | Measurement of FENa at baseline and after Acyl-Ghrelin injection |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Renal function | 5-6 Hours | Measurement of GFR, FEK and CH20 at baseline and after Acyl-Ghrelin injection |
| Water and sodium transport | 5-6 Hours | Measurement of u-AQP2, u-NCC , u-NKCC -2 and u-ENaC at baseline and after Acyl-Ghrelin |
| Vasoactive hormones | 5-6 Hours | Measurement of p-BNP , PRC, p-AngII , p-Aldo and p-AVP at baseline and after Acyl-Ghrelin |
| Blood pressure | 5-6 Hours | Measurement of Central blood pressure, PWV, AIx and Peripheral blood pressure at baseline and after Acyl-Ghrelin |
Countries
Denmark
Contacts
Primary ContactMy ES Malmberg
Outcome results
None listed