The Changes Within the Cells of the Drainage System of the Eye in Patients With Glaucoma

The Changes of Mitochondrial Dynamics and the Molecular Mechanisms in the Trabecular Meshwork of Patients With Glaucoma

Status

Withdrawn

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02524431

Enrollment

Registered

2015-08-14

Start date

2014-11-30

Completion date

2016-01-31

Last updated

2016-11-21

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Glaucoma

Keywords

Mitochondria, trabeculectomy, trabecular meshwork, Glaucoma surgery

Brief summary

The purpose of this study is to try to identify the cause of damage to the drainage system of the eye (the trabecular meshwork). Damage to this system may cause elevation in the pressure within the eye and thereby damage to the optic nerve and the vision.

Detailed description

During a routine trabeculectomy surgery, a corneo-scleral block that includes TM tissue will be collected at the operating room. This tissue is routinely removed during every trabeculectomy surgery. The tissue will be stored immediately in normal saline at 4 degrees Celsius, and walked directly to the Jefferson Center for Translational Medicine at Thomas Jefferson University by the Wills eye glaucoma research fellow. TM tissue will be identified using light microscope base on TM cell pigmentation. The ocular tissue will be fixed and placed in pre-cooled fixative on ice for 1 hour. The lengths of mitochondrial cross sections at the longest extent will be measured under electron microscopy (EM) in order to identify the mitochondrial dynamics. Quantitative Polymerase Chain Reaction (qPCR) will be done to identify proteins responsible for mitochondrial fusion.

Interventions

OTHERCollection of trabecular meshwork tissue during surgery

Tissue is collected during surgery at Wills Eye and then processed at thomas jefferson to identify the trabecular meshwork using light microscope. The ocular tissue will be fixed and the mitochondrial cross sections at the longest extent will be measured under electron microscopy (EM) in order to identify the mitochondrial dynamics. Quantitative Polymerase Chain Reaction (qPCR) will be done to identify proteins responsible for mitochondrial fusion.

Study design

Allocation

NON_RANDOMIZED

Intervention model

PARALLEL

Primary purpose

BASIC_SCIENCE

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

21 Years to 80 Years

Healthy volunteers

Inclusion criteria

* Minimum 2-year diagnosis of POAG * Moderate to advanced stage of the disease * DDLS stages 5 through 10 with visual field loss * Age between 21 and 80 years * Planned trabeculectomy with or without concomitant cataract surgery

Exclusion criteria

* Age ≤ 20 years old * Any other type of glaucoma other than POAG * Patients who had undergone selective laser trabeculoplasty (SLT) or argon laser trabeculoplasty (ALT) * Patients with history of ocular trauma * Patients with previous eye surgery will be excluded both from the study and the control groups

Design outcomes

Primary

Measure	Time frame
3-D EM tomography for mitochondria obtained from sample tissue	1 year

Secondary

Measure	Time frame
Gene expression of Drp1	1 year

Countries

United States

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026