Healthy
Conditions
Keywords
blueberry polyphenols, Polyphenol metabolites, endothelial function, Molecular mechanisms of action, Vascular function, Healthy men
Brief summary
Diets rich in fruits and vegetables have been linked to favorable cardiovascular outcomes in epidemiological studies. Dietary intervention studies with certain micronutrients have shown promising effects on surrogate parameters of vascular risk including blood pressure, endothelial function and cholesterol levels. Wild blueberries have gained attention due to their high content of phytochemicals and particularly anthocyanins. Whether wild blueberries can improve vascular function and health when given over relevant time periods and in relevant populations is not known. Therefore, it is the overall hypothesis of the study proposal that chronic consumption of wild blueberries can improve endothelial function, a prognostically validated surrogate of cardiovascular risk.
Interventions
DIETARY_SUPPLEMENTBlueberry Group
22 grams freeze-dried blueberry powder per day. Sustained intake (2x 11 grams daily over 1 month) of a drink consisting on freeze-dried wild blueberry powder dissolved in water
DIETARY_SUPPLEMENTPlacebo Group
Sustained intake (2x 11 grams daily over 1 month) of a drink consisting in placebo powder dissolved in water
Sponsors
Heinrich-Heine University, Duesseldorf
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
MALE
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* healthy male subjects without clinical signs or symptoms of cardiovascular disease * 18-70 years
Exclusion criteria
* cardiovascular disease * acute inflammation * cardiac arrhythmia * renal failure * heart failure (NYHA II-IV) * diabetes mellitus * C- reactive protein \> 1mg/dl * malignant disease
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Endothelial function | baseline and 1 month | Determination of endothelial function by Flow mediated dilation (FMD) 0 and 2 hours postconsumption |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Pulse wave velocity | baseline and 1 month | Measured by SphygmoCor 0 and 2 hours postconsumption |
| Blood pressure | baseline and 1 month | automatical measurements, 0 and 2 hours postconsumption |
| Blood lipids | baseline and 1 month | biochemical analysis, 0 and 2 hours postconsumption |
Other
| Measure | Time frame | Description |
|---|---|---|
| Plasma blueberry polyphenol metabolites | baseline and 1 month | measured by liquid chromotography- mass spectrometry (LC/MS) 0 and 2 hours postconsumption |
| Peripheral blood mononuclear cell-derived gene expression analysis | baseline and 1 month | exploratory measures; measured by Agilent Human GZ 4x44 v2 Microarray |
Countries
Germany
Outcome results
None listed