Dietary Modifications
Conditions
Keywords
Raspberries, Anthocyanins, Ellagitannins, Metabolites
Brief summary
Red raspberries are a rich source of (poly)phenolic compounds, the main components being anthocyanins and ellagitannins. There is growing evidence linking consumption of these compounds with beneficial effects on human health. However, the mechanisms involved remain poorly understood principally because of a limited understanding of the bioavailability of ellagitannins and anthocyanins. This study aims to explore the identity and amounts of the phenolic metabolites of anthocyanins and ellagitannins in human plasma and urine following acute ingestion of raspberries. For this purpose ten healthy volunteers were feed 300 g of blended raspberries containing in total 293 μmol anthocyanins and 250 μmol ellagitannins. All urine excreted over 48 h after the ingestion of raspberries was collected and blood samples were collected before (0 h) and after raspberry consumption up to 24 h. Metabolites were identified and quantified by ultra high performance liquid chromatography-mass spectrometry (UHPLC-MS).
Interventions
OTHERBioavailability of Raspberry polyphenols
Sponsors
University of Glasgow
National Processed Raspberry Council
University of Parma
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* in general good health
Exclusion criteria
* smoking * pregnancy * vegetarian * sustained use of nutritional supplements
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Changes in urinary anthocyanin and ellagitannin metabolite excretion after ingestion of 300g blended raspberries measured by ultra-high performance liquid chromatography-mass spectrometry | baseline excretion (12 h before intervention), and 0-4 h, 4-8 h, 8-24 h, 24-32 h, 32-48 h post ingestion | All urine excreted for 12 h prior to supplementation was collected as well as that excreted over 48 h after ingestion of raspberries. Urine samples were vortexed, centrifuged at 16110 g for 10 min at 5°C, and passed through 0.45 μm filter discs prior to the analysis of 5 μL aliquots by UHPLC-MS. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Changes in plasma concentration of anthocyanin and ellagitannin metabolites after ingestion of 300g blended raspberries measured by ultra-high performance liquid chromatography-mass spectrometry | 0 h, 0.5 h, 1 h, 1.5 h, 2 h, 3 h, 4 h, 5 h, 6 h, 8 h, 24 h post ingestion | Blood samples were collected in heparin tubes before and after raspberry consumption up to 24 h. Plasma samples were vortexed and 400 µL aliquots were mixed with 1 mL of 2% formic acid in acetonitrile. The samples were then vortexed and ultrasonicated for 10 min. After centrifugation at 16110 g for 10 min, supernatants were reduced to dryness in vacuo using a Speedvac concentrator and resuspended in 100 µL of methanol:water:formic acid (50:50:0.1, v/v/v) which was centrifuged at 16100 g for 10 min and 5 μL aliquots of the supernatant analysed by UHPLC-MS. |
Countries
Italy
Outcome results
None listed