Effect of Procedural Variables on Outcome of Surgical Treatment of Vitiligo

Effect of Procedural-Related Variables on Melanocyte-Keratinocyte Suspension Transplantation in Non-Segmental Stable Vitiligo: A Clinical and Immunohistochemical Study

Status

Completed

Phases

Phase 1

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02510651

Enrollment

Registered

2015-07-29

Start date

2010-01-31

Completion date

2014-06-30

Last updated

2015-07-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Vitiligo

Keywords

: non-segmental vitiligo, melanocyte-keratinocyte suspension, cryoblebbing, CO2 laser

Brief summary

ORSHFS grafts yielded lower cell counts than NCECS but the M-K ratio and resultant repigmentation did not show significant difference with better healing of the donor site. Compared to cryoblebs, CO2 gave more homogenous pigmentation. The acral sites showed better results using combination of donor NCECS and recipient cryoblebs.

Detailed description

Background: Melanocyte-keratinocyte suspension (M-K susp) is gaining popularity for vitiligo treatment. Few studies have addressed procedure-related variables. Objective: To assess the effect of different M-K susp procedure-related variables on the clinical outcome in stable vitiligo. Methods: This retrospective study included 40 cases with stable non-segmental vitiligo treated by M-K susp followed by narrow band- ultraviolet B phototherapy twice weekly. Donor site was either a skin graft in non-cultured epidermal cell suspension (NCECS) (34 cases) or hair follicle unit in Outer Root Sheath Hair Follicle Suspension (ORSHFS) (6 cases). Recipient site was prepared by either cryoblebbing (24 cases) or carbon dioxide (CO2)laser resurfacing (16 cases). Cell counts and viability were recorded in the cell suspensions, and tissue melanocytes and keratinocytes were examined by Melan A and Cytokeratin respectively. Assessment of repigmentation was done 18 months after the procedure.

Interventions

PROCEDUREMelanocyte-keratinocyte suspension

Treatment of non-segmental vitiligo by CO2 laser followed by melanocyte-keratinocyte suspension.

Study design

Allocation

Intervention model

SINGLE_GROUP

Primary purpose

TREATMENT

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Inclusion criteria

* Stable non-segmental vitiligo

Exclusion criteria

* Active vitiligo

Design outcomes

Primary

Measure	Time frame
The number of patients with satisfactory response after the surgical procedure for vitiligo.	one year

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026